LOCUS       V009326                 6332 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009326
VERSION     V009326
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Roh JY, Choi JY, Kang JN, Wang Y, Shim HJ, Liu Q, Tao X, Xu HG, Hyun
            JH, Woo SD, Jin BR, Je YH.
  TITLE     Simple purification of a foreign protein using polyhedrin fusion in
            a baculovirus expression system
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (8), 1522-1526 (2010)
   PUBMED   20699589
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Je YH, Roh JY, Choi JY, Kang JN.
  TITLE     Simple purification of a foreign protein using polyhedrin fusion in
            a baculovirus expression system
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6332)
  AUTHORS   Je YH, Roh JY, Choi JY, Kang JN.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2010) Agricultural Biotechnology, Seoul National
            University, #200 Room 6117, San56-1, Sillim9-dong, Gwanak-gu, Seoul
            151-921, Republic of Korea
REFERENCE   4  (bases 1 to 6332)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci.
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "8";
            pages: "1522-1526"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-FEB-2010) Agricultural Biotechnology, Seoul National University,
            #200 Room 6117, San56-1, Sillim9-dong, Gwanak-gu, Seoul 151-921,
            Republic of Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        1158..1249
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1258..1992
                     /gene="PH"
                     /label="Polyhedrin"
                     /note="Polyhedrin from Autographa californica nuclear
                     polyhedrosis virus. Accession#: P04871"
     CDS             2026..2040
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     misc_recomb     2131..3488
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     rep_origin      3635..4090
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4372..4476
                     /label="AmpR promoter"
     CDS             4477..5334
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5508..6096
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatctatg
     1261 ccggattatt cataccgtcc caccatcggg cgtacctacg tgtacgacaa caagtactac
     1321 aaaaatttag gtgccgttat caagaacgct aagcgcaaga agcacttcgc cgaacatgag
     1381 atcgaagagg ctaccctcga ccccctagac aactacctag tggctgagga tcctttcctg
     1441 ggacccggca agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt taaacccgac
     1501 acgatgaagc ttgtcgttgg atggaaagga aaagagttct acagggaaac ttggacccgc
     1561 ttcatggaag acagcttccc cattgttaac gaccaagaag tgatggatgt tttccttgtt
     1621 gtcaacatgc gtcccactag acccaaccgt tgttacaaat tcctggccca acacgctctg
     1681 cgttgcgacc ccgactatgt acctcatgac gtgattagga tcgtcgagcc ttcatgggtg
     1741 ggcagcaaca acgagtaccg catcagcctg gctaagaagg gcggcggctg cccaataatg
     1801 aaccttcact ctgagtacac caactcgttc gaacagttca tcgatcgtgt catctgggag
     1861 aacttctaca agcccatcgt ttacatcggt accgactctg ctgaagagga ggaaattctc
     1921 cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact
     1981 ggtccggcgt atgaagagga agaggaagag aacaataacg atgacgatga cgatgacaag
     2041 ggtcaatcac tagtgaattc gagctcggta ccagatcttc tagattcgaa ctcgaggcct
     2101 gcagggcggc cgcttaatta attgatccgg gttattagta catttattaa gcgctagatt
     2161 ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca ttaaatttat
     2221 aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga
     2281 atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt tcaaacaagg
     2341 gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac gccacaaaac
     2401 ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta
     2461 ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca tcactgtcgt
     2521 tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ttggacatat ttaacatcgg
     2581 gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg ttagaagttg
     2641 cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg gctaacacgt
     2701 ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg cgtttttggg
     2761 cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa agcgatggtg
     2821 caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt ggagctgatg
     2881 ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg
     2941 gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac acagtcggca
     3001 cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg agacgagtgc
     3061 gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa ggtgcagcgg
     3121 gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat ggtggtggtg
     3181 gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt gccgccggta
     3241 taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca ggcgccgctg
     3301 gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc aattcaatat
     3361 tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg ctatcgttta
     3421 ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga gattgtctca
     3481 agctcggatc gatcccgcac gccgataaca agccttttca tttttactac agcattgtag
     3541 tggcgagaca cttcgctgtc gtcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3601 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     3661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3841 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3961 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4081 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4141 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4201 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4261 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4321 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4381 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4441 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4501 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4561 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4621 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4681 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4741 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4801 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4861 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4921 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4981 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5041 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5101 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5161 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5221 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5281 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5341 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5401 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5461 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5521 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5581 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5641 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5701 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5761 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5821 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5881 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5941 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6001 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6061 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6121 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6181 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6241 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6301 ctccccgcgc gttggccgat tcattaatgc ag
//
