pAW016 vector (V009388)

LOCUS       V009388                 7352 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009388
VERSION     V009388
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Wilson AC, Perego M, Hoch JA.
  TITLE     New transposon delivery plasmids for insertional mutagenesis in
            Bacillus anthracis
  JOURNAL   J. Microbiol. Methods 71 (3), 332-335 (2007)
   PUBMED   17931726
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Wilson AC, Perego M, Hoch JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2007) Department of Molecular and Experimental,
            The Scripps Research Institute, 10550 North Torrey Pines Road, La
            Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 7352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2007"; volume: "71"; issue: "3"; pages:
            "332-335"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-SEP-2007) Department of Molecular and Experimental, The Scripps
            Research Institute, 10550 North Torrey Pines Road, La Jolla, CA
            92037, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..43
                     /mobile_element_type="transposon:mini-Tn10"
                     /label="n10"
     rep_origin      complement(320..908)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1204..1983
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     mobile_element  complement(2308..2351)
                     /mobile_element_type="transposon:mini-Tn10"
                     /label="n10"
     CDS             complement(2543..3190)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(3908..3919)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             4531..5229
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /protein_id="ABV70025.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            4531..5229
                     /gene="repA"
                     /label="repA"
     CDS             complement(5940..6878)
                     /codon_start=1
                     /product="transposase"
                     /label="transposase"
                     /protein_id="ABV70026.1"
                     /translation="MPIVLVDWSDIREQKRLMVLRASVALHGRSVTLYEKAFPLSEQCS
                     KKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGA
                     ENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPS
                     PKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRT
                     SSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRH
                     SGYTITREDLLVAATLLAQNLFTHGYALGKL"
     RBS             7155..7166
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     protein_bind    complement(7207..7223)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."