LOCUS       V009388                 7352 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009388
VERSION     V009388
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Wilson AC, Perego M, Hoch JA.
  TITLE     New transposon delivery plasmids for insertional mutagenesis in
            Bacillus anthracis
  JOURNAL   J. Microbiol. Methods 71 (3), 332-335 (2007)
   PUBMED   17931726
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Wilson AC, Perego M, Hoch JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2007) Department of Molecular and Experimental,
            The Scripps Research Institute, 10550 North Torrey Pines Road, La
            Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 7352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2007"; volume: "71"; issue: "3"; pages:
            "332-335"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-SEP-2007) Department of Molecular and Experimental, The Scripps
            Research Institute, 10550 North Torrey Pines Road, La Jolla, CA
            92037, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..43
                     /mobile_element_type="transposon:mini-Tn10"
                     /label="n10"
     rep_origin      complement(320..908)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1204..1983
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     mobile_element  complement(2308..2351)
                     /mobile_element_type="transposon:mini-Tn10"
                     /label="n10"
     CDS             complement(2543..3190)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(3908..3919)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             4531..5229
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /protein_id="ABV70025.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            4531..5229
                     /gene="repA"
                     /label="repA"
     CDS             complement(5940..6878)
                     /codon_start=1
                     /product="transposase"
                     /label="transposase"
                     /protein_id="ABV70026.1"
                     /translation="MPIVLVDWSDIREQKRLMVLRASVALHGRSVTLYEKAFPLSEQCS
                     KKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGA
                     ENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPS
                     PKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRT
                     SSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRH
                     SGYTITREDLLVAATLLAQNLFTHGYALGKL"
     RBS             7155..7166
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     protein_bind    complement(7207..7223)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcactgatga atcccctaat gattttggta aaaatcatta agttaaggtg gatacacatc
       61 ttgtcatatg atcccggatc cccctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      121 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatgcg
      661 gactggcagc agccactggt aacaggatta cgagagcgag gtatgtaggc ggtgctacag
      721 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      841 ccaccgctgg tagcggtggt ttttttgttt cgaagcagca gattacgcgc agaaaaaaag
      901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttga
     1021 ctctagagga tcgatctgta taataaagaa taattattaa tctgtagaca aattgtgaaa
     1081 ggatgtactt aaaccgtaac ggtcagcttt attgaacagt aatttaagta tatgtccaat
     1141 ctagggtaag taaattgagt atcaatataa actttatatg aacataatca acgaggtgaa
     1201 atcatgacca atttgattaa cggaaaaata ccaaatcaag cgattcaaac attaaaaatc
     1261 gtaaaagatt tatttggaag ttcaatagtt ggagtatatc tatttggttc agcagtaaat
     1321 ggtggtttac gcattaacag cgatgtagat ggtctagtcg tcgtgaatca tagtttacct
     1381 caattaactc gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa gattggaaat
     1441 acggattctg ttagaccact tgaagttacg gttataaata ggagtgaagt tgtcccttgg
     1501 caatatcctc caaaaagaga atttatatac ggtgagtggc tcaggggtga atttgagaat
     1561 ggacaaattc aggaaccaag ctatgatcct gatttggcta ttgttttagc acaagcaaga
     1621 aagaatagta tttctctatt tggtcctgat tcttcaagta tacttgtctc cgtacctttg
     1681 acagatattc gaagagcaat taaggattct ttgccagaac taattgaggg gataaaaggt
     1741 gatgagcgta atgtaatttt aaccgtagct gcaatgtggc aaacagtgac tactggtgaa
     1801 attacctcga aagatgtcgc tgcagaatgg gctatacctc ttttacctaa agagcatgta
     1861 actttactgg atatagctag aaaaggctat cggggagagt ggtatgataa gtgggaagga
     1921 ctatattcaa aggtgaaagc actcgttaag tatatgaaaa attctataga aacttctctc
     1981 aattaggcta attttattcg aataacaggt gcttactttt aaaactactg atttattgat
     2041 aaatattgaa caatttttgg gaagaataaa gcgtcctctt gtgaaattag agaacgcttt
     2101 attactttaa tttagtgaaa caatttgtaa ctattgaaaa tagaaagaaa ttgttccttg
     2161 catagtttat taatattagt gacatttgca tgcttcaaag cctgtcggaa ttggttttta
     2221 ctgaaaagaa cacgatattc agcgtttacc cacttataaa caaaagatcg gatccgggat
     2281 catatgacaa gatgtgtatc caccttaact taatgatttt taccaaaatc attaggggat
     2341 tcatcagtgc tcagggtcaa cgagaattaa cattccgtca ggaaagctta tgatgatgat
     2401 gtgcttaaaa acttactcaa tggctggttt atgcattcga tgatctggag ctgtaatata
     2461 aaaaccttct tcaactaacg gggcaggtta gtgacattag aaaaccgact gtaaaaagta
     2521 cagtcggcat tatctcatat tataaaagcc agtcattagg cctatctgac aattcctgaa
     2581 tagagttcat aaacaatcct gcatgataac catcacaaac agaatgatgt acctgtaaag
     2641 atagcggtaa atatattgaa ttacctttat taatgaattt tcctgctgta ataatgggta
     2701 gaaggtaatt actattatta ttgatattta agttaaaccc agtaaatgaa gtccatggaa
     2761 taatagaaag agaaaaagca ttttcaggta taggtgtttt gggaaacaat ttccccgaac
     2821 cattatattt ctctacatca gaaaggtata aatcataaaa ctctttgaag tcattcttta
     2881 caggagtcca aataccagag aatgttttag atacaccatc aaaaattgta taaagtggct
     2941 ctaacttatc ccaataacct aactctccgt cgctattgta accagttcta aaagctgtat
     3001 ttgagtttat cacccttgtc actaagaaaa taaatgcagg gtaaaattta tatccttctt
     3061 gttttatgtt tcggtataaa acactaatat caatttctgt ggttatacta aaagtcgttt
     3121 gttggttcaa ataatgatta aatatctctt ttctcttcca attgtctaaa tcaattttat
     3181 taaagttcat ttgatatgcc tcctaaattt ttatctaaag tgaatttagg aggcttactt
     3241 gtctgctttc ttcattagaa tcaatccttt tttaaaagtc aatattactg taacataaat
     3301 atatatttta aaaatatccc actttatcca attttcgttt gttgaactaa tgggtgcttt
     3361 agttgaagaa taaagaccac attaaaaaat gtggtctttt gtgttttttt aaaggatttg
     3421 agcgtagcga aaaatccttt tctttcttat cttgataata agggtaacta ttgccggagc
     3481 tctgataaat atgaacatga tgagtgatcg ttaaatttat actgcaatcg gatgcgatta
     3541 ttgaataaaa gatatgagag atttatctaa tttctttttt cttgtaaaaa aagaaagttc
     3601 ttaaaggttt tatagttttg gtcgtagagc acacggttta acgacttaat tacgaagtaa
     3661 ataagtctag tgtgttagac tttatgaaat ctatatacgt ttatatatat ttattatccg
     3721 attttttatt aaaacgtctc aaaatcgttt ctgagacgtt ttagcgttta tttcgtttag
     3781 ttatcggcat aatcgttaaa acaggcgtta tcgtagcgta aaagcccttg agcgtagcgt
     3841 ggctttgcag cgaagatgtt gtctgttaga ttatgaaagc cgatgactga atgaaataat
     3901 aagcgcagcg cccttctatt tcggttggag gaggctcaag ggagtatgag ggaatgaaat
     3961 tccctcatgg gtttgatttt aaaaattgct tgcaattttg ccgagcggta gcgctggaaa
     4021 atttttgaaa aaaatttgga atttggaaaa aaatgggggg aaaggaagcg aattttgctt
     4081 ccgtactacg accccccatt aagtgccgag tgccaatttt tgtgccaaaa acgctctatc
     4141 ccaactggct caagggttta aggggttttt caatcgccaa cgaatcgcca acgttttcgc
     4201 caacgttttt tataaatcta tatttaagta gctttattgt tgtttttatg attacaaagt
     4261 gatacactaa ctttataaaa ttatttgatt ggagtttttt aaatggtgat ttcagaatcg
     4321 aaaaaaagag ttatgatttc tctgacaaaa gagcaagata aaaaattaac agatatggcg
     4381 aaacaaaaag gtttttcaaa atctgcggtt gcggcgttag ctatagaaga atatgcaaga
     4441 aaggaatcag aacaaaaaaa ataagcgaaa gctcgcgttt ttagaaggat acgagttttc
     4501 gctacttgtt tttgataagg taattatatc atggctatta aaaatactaa agctagaaat
     4561 tttggatttt tattatatcc tgactcaatt cctaatgatt ggaaagaaaa attagagagt
     4621 ttgggcgtat ctatggctgt cagtccttta cacgatatgg acgaaaaaaa agataaagat
     4681 acatggaata gtagtgatgt tatacgaaat ggaaagcact ataaaaaacc acactatcac
     4741 gttatatata ttgcacgaaa tcctgtaaca atagaaagcg ttaggaacaa gattaagcga
     4801 aaattgggga atagttcagt tgctcatgtt gagatacttg attatatcaa aggttcatat
     4861 gaatatttga ctcatgaatc aaaggacgct attgctaaga ataaacatat atacgacaaa
     4921 aaagatattt tgaacattaa tgattttgat attgaccgct atataacact tgatgaaagc
     4981 caaaaaagag aattgaagaa tttactttta gatatagtgg atgactataa tttggtaaat
     5041 acaaaagatt taatggcttt tattcgcctt aggggagcgg agtttggaat tttaaatacg
     5101 aatgatgtaa aagatattgt ttcaacaaac tctagcgcct ttagattatg gtttgagggc
     5161 aattatcagt gtggatatag agcaagttat gcaaaggttc ttgatgctga aacgggggaa
     5221 ataaaatgac aaacaaagaa aaagagttat ttgctgaaaa tgaggaatta aaaaaagaaa
     5281 ttaaggactt aaaagagcgt attgaaagat acagagaaat ggaagttgaa ttaagtacaa
     5341 caatagattt attgagagga gggattattg aataaataaa agccccctga cgaaagtcga
     5401 agggggtttt tattttggtt tgatgttgcg attaatagca atacaattgc aataaacaaa
     5461 atgatcttcc ttcaggttat gaccatctgt gccagttcgt aatgtctggt caactttccg
     5521 actctgagaa acttctggaa tcgctagaga atttctggaa tgggattcag gagtggacag
     5581 aacgacacgg atatatagtg gatgtgtcaa aacgcatacc attttgaacg atgacctcta
     5641 ataattgtta atcatgttgg ttacgtattt attaacttct cctagtatta gtaattatca
     5701 tggctgtcat ggcgcattaa cggaataaag ggtgtgctta aatcgggcca ttttgcgtaa
     5761 taagaaaaag gattaattat gagcgaattg aattaataat aaggtaatag atttacatta
     5821 gaaaatgaaa ggggatttta tgcgtgagaa tgttacagtc tatcccgtca ggcagccatc
     5881 ggaagctgtg gtatggctgt gcaggtcgta aatccactgc ataatttaga gcgatcccct
     5941 cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc
     6001 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
     6061 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
     6121 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
     6181 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
     6241 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
     6301 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc
     6361 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
     6421 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga
     6481 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
     6541 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
     6601 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
     6661 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
     6721 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
     6781 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc
     6841 tcacgaatat cagaccagtc aacaagtaca atgggcatcg tattgcgcga acagataaag
     6901 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
     6961 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
     7021 tcggtaagag tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg
     7081 agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt
     7141 tcgcaaatag ttaatttctc ctctttaatg aattcgaaac catttgatcc tgtttcctgt
     7201 gtgaaattgt tatccgctca caattccaca cattatacga gccgatgatt aatnggcaac
     7261 agctcatttc agaatatttg ccagaaccgt tatgatgtcg gcgcaaaaaa cattatccag
     7321 aacgggagtg cgccttgagc gacacgaatt at
//
