pAV007 vector (V009392)

LOCUS       40924_5084        3452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAV007, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   White KP, Victorsen A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2013) Human Genetics, The University of Chicago, 
            5801 South Ellis Avenue, Chicago, IL 60637, USA
REFERENCE   2  (bases 1 to 3452)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2013) Human Genetics, The University of Chicago, 5801 South 
            Ellis Avenue, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..22
                     /label=forward recombineering primer binding site
                     /note="forward recombineering primer binding site"
     CDS             37..753
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     CDS             778..795
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             817..840
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             841..861
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             904..927
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             928..951
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             952..972
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    973..1044
                     /note="BLRP; biotin ligase (birA) recognition peptide"
     misc_feature    1064..1649
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      1652..1657
                     /gene="aphA"
                     /regulatory_class="minus_35_signal"
     regulatory      1672..1680
                     /gene="aphA"
                     /regulatory_class="minus_10_signal"
     CDS             1731..2531
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(2748..3136)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            3296..3405
                     /label=oriT
                     /note="incP origin of transfer"