LOCUS       40924_5084        3452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAV007, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   White KP, Victorsen A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2013) Human Genetics, The University of Chicago, 
            5801 South Ellis Avenue, Chicago, IL 60637, USA
REFERENCE   2  (bases 1 to 3452)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2013) Human Genetics, The University of Chicago, 5801 South 
            Ellis Avenue, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..22
                     /label=forward recombineering primer binding site
                     /note="forward recombineering primer binding site"
     CDS             37..753
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     CDS             778..795
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             817..840
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             841..861
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             904..927
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             928..951
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             952..972
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    973..1044
                     /note="BLRP; biotin ligase (birA) recognition peptide"
     misc_feature    1064..1649
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      1652..1657
                     /gene="aphA"
                     /regulatory_class="minus_35_signal"
     regulatory      1672..1680
                     /gene="aphA"
                     /regulatory_class="minus_10_signal"
     CDS             1731..2531
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(2748..3136)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            3296..3405
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 ggaggttccg gtggaagcgg aggtagcggc ggcagcatgg tgtccaaggg cgaggagctg
       61 ttcaccggcg tggtgcccat cctggtggag ctggatggcg acgtgaacgg ccacaagttc
      121 agcgtgcgcg gcgagggcga gggcgacgcc accaacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg tgaccaccct gacctacggc
      241 gtgcagtgct tcagccgcta ccccgatcac atgaagcagc acgatttctt caagagcgcc
      301 atgcccgagg gctacgtgca ggagcgcacc atcagcttca aggatgacgg cacctacaag
      361 acccgcgccg aggtgaagtt cgagggcgat accctggtga accgcatcga gctgaagggc
      421 atcgatttca aggaggatgg caacatcctg ggccacaagc tggagtacaa cttcaacagc
      481 cacaacgtgt acatcaccgc cgataagcag aagaacggca tcaaggccaa cttcaagatc
      541 cgccacaatg tggaggatgg ctccgtgcag ctggccgatc actaccagca gaacaccccc
      601 atcggcgacg gcccagtgct gctgcccgat aaccactacc tgagcaccca gagcgtgctg
      661 tccaaggacc ccaacgagaa gcgcgatcac atggtgctgc tggagttcgt gaccgccgcc
      721 ggcatcaccc tgggcatgga tgagctgtac aaggagagca gcggcagctt cctgaactgc
      781 tgcccaggat gctgcatgga accgggaagc ggcaactggt cccaccccca gttcgagaag
      841 gaaaacctgt acttccagag cgattacaag gatcacgacg gcgactacaa ggaccacgat
      901 attgattaca aggacgatga cgacaagctg gaggtgctgt tccagggccc cgagaacctg
      961 tacttccagg gcatggccag cagcctgcgc cagatcctgg atagccagaa gatggagtgg
     1021 cgcagcaacg ccggcggcag ctgaacgaca agtaagaatt ccgcccctct ccctcccccc
     1081 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     1141 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     1201 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     1261 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     1321 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     1381 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     1441 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     1501 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     1561 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     1621 aaaaacacga tgataatatg gccacaacct gttacattgc acaagataaa aatatatcat
     1681 cacgaacagt aaaactgtct gcttacataa acagtaatac aaggggtgtt atgggatcgg
     1741 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     1801 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1861 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1921 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     1981 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     2041 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     2101 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     2161 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     2221 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     2281 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     2341 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     2401 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     2461 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     2521 acgagttctt ctgaatcttt ctagaagatc tcctacaatt tcaatattat tgaagcattt
     2581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2641 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctatccg cttcctttag
     2701 cagcccttgc gccctgagtg cttgcggcag cgtgaagcta attcccatgt cagccgttaa
     2761 gtgttcctgt gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca
     2821 tccctggctt gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct
     2881 tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt
     2941 ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg
     3001 agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta
     3061 aacatgagag cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac
     3121 tgctgatctt cagatcctct acgccggacg catcgtggcc gttttccgct gcataaccct
     3181 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     3241 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     3301 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     3361 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa
     3421 cagatgaggg caactcgagt ttttcagcaa ga
//
