pAV-UCSF vector (V009394)

LOCUS       V009394                11534 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009394
VERSION     V009394
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Greninger AL, Knudsen GM, Betegon M, Burlingame AL, Derisi JL.
  TITLE     The 3A Protein from Multiple Picornaviruses Utilizes the Golgi
            Adaptor Protein ACBD3 To Recruit PI4KIIIbeta
  JOURNAL   J. Virol. 86 (7), 3605-3616 (2012)
   PUBMED   22258260
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Greninger AL, DeRisi JL, Knudsen GM, Miguel B, A B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Biochemistry, UCSF, 1700 4th Street, San
            Francisco, CA 94110, USA
REFERENCE   3  (bases 1 to 11534)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11534)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2012"; volume: "86"; issue: "7"; pages: "3605-3616"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-DEC-2011) Biochemistry, UCSF, 1700 4th Street, San Francisco, CA
            94110, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..743
     CDS             744..8039
                     /note="Genome polyprotein from Aichi virus (strain
                     Human/A846/88/1989). Accession#: O91464"
                     /label="Genome polyprotein"
     3'UTR           8043..8319
     polyA_site      8279
     primer_bind     complement(8333..8349)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      8562..9017
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9299..9403
                     /label="AmpR promoter"
     CDS             9404..10261
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10435..11023
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    11311..11332
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11347..11377
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11385..11401
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11409..11425
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        11454..11472
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"