LOCUS       V009394                11534 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009394
VERSION     V009394
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Greninger AL, Knudsen GM, Betegon M, Burlingame AL, Derisi JL.
  TITLE     The 3A Protein from Multiple Picornaviruses Utilizes the Golgi
            Adaptor Protein ACBD3 To Recruit PI4KIIIbeta
  JOURNAL   J. Virol. 86 (7), 3605-3616 (2012)
   PUBMED   22258260
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Greninger AL, DeRisi JL, Knudsen GM, Miguel B, A B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Biochemistry, UCSF, 1700 4th Street, San
            Francisco, CA 94110, USA
REFERENCE   3  (bases 1 to 11534)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11534)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2012"; volume: "86"; issue: "7"; pages: "3605-3616"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-DEC-2011) Biochemistry, UCSF, 1700 4th Street, San Francisco, CA
            94110, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..743
     CDS             744..8039
                     /note="Genome polyprotein from Aichi virus (strain
                     Human/A846/88/1989). Accession#: O91464"
                     /label="Genome polyprotein"
     3'UTR           8043..8319
     polyA_site      8279
     primer_bind     complement(8333..8349)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      8562..9017
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9299..9403
                     /label="AmpR promoter"
     CDS             9404..10261
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10435..11023
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    11311..11332
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11347..11377
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11385..11401
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11409..11425
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        11454..11472
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgaaaaggg ggtggggggg cctcggcccc ctcaccctct tttccggtgg tctggtcccg
       61 gaccaccgtt actccattca gcttcttcgg aacctgttcg gaggaattaa acgggcaccc
      121 atactccccc cacccccctt ttgtaactaa gtatgtgtgc tcgtgatctt gactcccacg
      181 gaacggaccg atccgttggt gaacaaacag ctaggtccac atcctccctt cccctgggag
      241 ggcccccgcc ctcccacatc ctccccccag cctgacgtat cacaggctgt gtgaagcccc
      301 cgcgaaagct gctcacgtgg caattgtggg tccccccttc atcaagacac caggtctttc
      361 ctccttaagg ctagccccgg cgtgtgaatt cacgttgggc aactagtggt gtcactgtgc
      421 gctcccaatc tcggccgcgg agtgctgttc cccaagccaa acccctggcc cttcactatg
      481 tgcctggcaa gcatatctga gaaggtgttc cgctgtggct gccaacctgg tgacaggtgc
      541 cccagtgtgc gtaaccttct tccgtctccg gacggtagtg attggttaag atttggtgta
      601 aggttcatgt gccaacgccc tgtgcgggat gaaacctcta ctgccctagg aatgccaggc
      661 aggtacccca cctccgggtg ggatctgagc ctgggctaat tgtctacggg tagtttcatt
      721 tccaatcctt ttatgtcgga gtcatggctg caacacgggt ttcacgatct gtgctcgctg
      781 ctgtggctca ctctgctgct caccgcacct accacactgt ccttgactgc tatgataggc
      841 tttacctcaa caccaacccc catctctctt atccccttcc caaaaactct tccttccctt
      901 gcccgttctg tcagtacgac gagcagaatg aggtcctctc cccagagtct ctccgtggcg
      961 agggagctga accctgctgg aagtgctcac aggacaaacc ccgccgcaag tacaacacca
     1021 cccctcctga agactggctc tatgattctg atgtccaatc ctggttctat cctgaaacat
     1081 actactctga ccttcagcaa aagttctttg acaaattggc tctcctctct cttccaggtg
     1141 cctaccaagc aaagaccccg gaggagcgtg ccctcgcggg cgcgctcacc caactcctca
     1201 acttcccctc caccccccct ctcacactcc ctaccactaa cctccaacgg caaggcaact
     1261 cggtcaccaa catctacggt aacggcaaca acgtcaccac tgacgttggc gccaacggtt
     1321 gggcccccac ggtctcaact ggcctaggtg atggtcccgt ctctgcctcc gccgactccc
     1381 tccccgggcg atccggaggt gcctcctccg agaaaaccca caccgtttcc ggctcctcca
     1441 acaaagtcgg atcccgcttt tccaagtggt gggaaccagc agccgcccgc gcttcggaat
     1501 ccgccaccga ctcggctatc gagggaatcg atgctgccgg gaaggccgcc tccaaggcca
     1561 tcacgcggaa gctggaccgc cccgccgccc cctcctcaac cgccaatccc caaccatctc
     1621 tcatcgctct caacccttct gccacgcaat ccggcaacgc ttccatcttg accggatcaa
     1681 ctgctccctc cctcctcgcc taccccaccg ccacccctgt tccccttccc aaccccgatg
     1741 aaccctccca acccggacca tccggcgacc gcacctggtt gttggataca gtcacctggt
     1801 cccaggagtt cacccgcggc tggaacatcg caggctccaa tggcatgcaa tggaccggcc
     1861 tggaatccct tatcttccct gtctccactg acaccaattg gacctccacc tccagtccta
     1921 ccgcatatcc cctccctttc tccttcgtgc gtgcttaccc agactcctcc tgggctgcca
     1981 tgtacaacac ccactccatg tggaattgcg gttggcgcgt tcaagtgacc gtcaacggct
     2041 cacagttcca tgccggagcc cttatcctct acatggtccc tgaagccacc acccatgcca
     2101 tccaaacggc tcgggacaac gctggctttg tcttccccta tgtcatcctc aatctctatg
     2161 aaagcaacac cgctaccata gaggtcccct acatctcccc cacccccaat acctcgagtg
     2221 gactccacgc cccgtggacg ttctacctcc aggtgctctc acccctcaat cctccccctt
     2281 ctctccccac ttccttatcc tgctctatct atgtcacccc cgttgactcc tctttccacg
     2341 gtctccgcta cctcgcccca cagcactgga agactcgcgc cgtcccaggt gcgggcactt
     2401 tcggatctgc cgtggctggc caggaactcc ctctctgtgg agtccgtgcc tactaccccc
     2461 ccaatgccta catccctgcc caggtacgcg actggcttga gttcgcccac cggcctggtc
     2521 tcatggccac agtgccgtgg accatggcgg acgagccggc cgagcgcctc ggtatcttcc
     2581 ccgtctctcc cagtgctatc gctgggactg gcgcccccat ctcctatgtt atttctctct
     2641 tctcccagtg gcgcggtgaa ctcgccgccc acctcctctt cactggctcc gctcagcact
     2701 acggccgcct ggtcgtgtgc tacactcctg ctgcccccca acccccctcc accatgcagg
     2761 aagcgatgcg cggaacatac accgtatggg acgtcaacgc tgcctcaacc ctggagttca
     2821 ccatcccctt catctctaac tcctactgga aaacagtgga cgttaacaac cccgacgctc
     2881 tgctgtccac caccggctat gtttccatct gggttcaaaa cccgctagtc ggaccccaca
     2941 ccgcccctgc gtcggccctc gtgcaggcat tcatctccgc aggtgaatcc ttcaacgtgc
     3001 gactgatgca aaaccccgcc ctcacttccc aaaccctcac cgaagacctc gatgcacccc
     3061 aagacaccgg gaacattgag aatggtgcgg ctgacaactc cccgcagccc cgcaccacct
     3121 tcgactacac cggaaacccc ctccctccag acaccaaact ggaaaacttc ttctccttct
     3181 accgcttgct gcccatgggt ggcagcggtg ctccatctct ttctttcccc gctgacgaag
     3241 gcacaatcat cccactcaac ccaatcaatt ggctcaaagg ggcggacgtc tccgggatcg
     3301 ctgcgatgct atcgtgcttc acgtacatcg ctgcggacct gcgaatcacg ctccgcttct
     3361 ccaacccaaa tgacaaccct gccaccatgc ttgttgcctt cgctccccct ggcgccacca
     3421 tccccctcaa acccacgcgc caaatgctct ccaacttcta catggcggag gtcccagtct
     3481 ccgctgccac ctccaccatg gtctctttct ccatccccta cacctccccc ctctctgcca
     3541 tccccacctc atacttcgga tgggaggact ggtcaggtac caactttggc cagctctcct
     3601 ccgggtcatg gggaaacctc atgctcatcc cctctctctc tgtcgactct gctatcccct
     3661 tcgacttcca gctctcttgc tgggtcgcct tcggcaactt caaagcctgg gtcccccgcc
     3721 cacctccacc tctccccccc ctccccaccc ctgccgcgaa cgcggaacgc acagtggccg
     3781 tgatcaagca gggcgccgcc tcagcgactc ccgatgtgga ccccgacgat cgggtctaca
     3841 tcgtgcgcgc gcagcgcccc acctacgtcc actgggccat ccgcaaggtg gcccccgacg
     3901 gcagtgccaa acagatctcg ctgtcccgca gcgggatcca ggcactggtc gccctcgaac
     3961 cccccgaggg tgaaccttac ctcgaaattc tcccctccca ttggaccctt gctgaactcc
     4021 aacttggcaa caagtgggag tacagcgcca ccaacaactg tacccacttc gtcagctcca
     4081 tcactggcga atccctcccc aacaccggct tctccctggc cctcggaatc ggtgccctca
     4141 ccgccatcgc agcctcggct gctgttgccg tgaaagcgct cccaggcatc aggcgacagg
     4201 gtctcctcac cctctctgcc gataccgaga ccaaccagac cctcaacaag atcactgaat
     4261 ctgtcaacca agctgcccag gtcgtctccc aattcgatct gagcggccct gcaaattctg
     4321 tctcccttgc tgcttctgac atccgcgagg ctgcccacaa agtggcctcc tccctcaatg
     4381 gcttcactga cgtcatcgct gacatcaaag attccctgtt caccagggtg tctgacgctg
     4441 tcgagagcgg tgtcgccact ttcctcactt ggctcgtcaa actctttggc tacctccttg
     4501 ttctctttgg ctctccaacc cccatgtcca tctccggtct cctcgtcatc atctgtgccg
     4561 accttgcccc ccacgctcgt gaatttttca ccgcctctgg aaatgtcctc tcctcactct
     4621 actactggat tgcctcaaaa ttaggcctgt ccgtcacgcc ccaagagtgt gaacgggcaa
     4681 cccttgaacc tcaagggctc aaagacttca acgacggcgc actcgcaatg cgcaacgtcg
     4741 agtggattgg tgagaccgcc tggaaatggg cccaccgcct ccttgactgg atccgcggta
     4801 aggccaagac agacccccag gccaaactcg ccgacgtcca cgatgaaatc atgctccact
     4861 actcagactc catattggct ctcggatctg agaaactccc cattgatcac atcactaaat
     4921 ccatctcccg ctgccgtgaa ttggtctcca tcgcccagga ggccaaatcc ggccctcact
     4981 cttccttcct caaccaagca atcaaaaact acacccttgc catctcccaa caccgcaaat
     5041 gccagacagg ccccaggcct gaacccgtgg tggtctacct ctacggaccc cccggcactg
     5101 gcaaatccct ccttgcttca ctcctcgctc aaaccctttc ccagcgtctt gctgggaccc
     5161 ccgatgacgt ctactccccc tcctccgcct cctgtgagta cttcgatggg tacacgggtc
     5221 aaaccgtgca ctttatcgat gacatcgggc aggatccgga ggggcgggac tgggctaatt
     5281 tcccaaacct cgtctcctct gcccccttca ttgtcccaat ggcctccctc gaggagaagg
     5341 gcacccacta cacctccaag gtcatcgtgg ttacctccaa cttccacgag cctaacgagc
     5401 gcgccgcgcg ctcgatgggc gctcttcgac gccgcgtcca cctgcggatc aacgtcacct
     5461 caaatggagt gccatttgac cctaccaatg ccctcaaccc catccccgga acccaatcta
     5521 aatacttcac cgcccaaaca cctctcaccc tattccagtc caacacggtc cgcctggatc
     5581 gtgactccat ctggacccct accttcacca acatggacga gttggtggac gcgattgtga
     5641 cccgtctcga tcgctccacc ggcgtgtcca attccctcgc ctccctcatc agacgccagg
     5701 gcaaccgggt catcgacgcg gaacccaggg aaatccccct ggaatacgcg gatgatctcc
     5761 tggaagccat ggcgcaccac cgccctgtac catgctccct tggcctctcc caagccatcg
     5821 caaacaacac ccccatccaa caaatctctg aaaccttctg gaagtaccgc aaacccatct
     5881 tcacttgcac cactttcctc gctgtcctcg gtttcctctg ctctgtcatc cctcttgctc
     5941 gctccctgtg gaagtccaaa caggacacgc cacaggaacc tcaagctgcc tactctgcca
     6001 tctcccacca gaaacccaag cccaagtccc agaaacccgt ccccactcgt cacatccagc
     6061 gccagggaat ctcccctgct gtccccggca tctccaacaa tgtcgtccat gtcgagtccg
     6121 gcaacggtct caacaagaac gtcatgtctg gcttctacat cttctcccgc ttccttctgg
     6181 ttcctaccca cctccgtgaa ccccaccaca ccactctcac ggtgggcgcc gacacctacg
     6241 actgggccac cctccagacg caggagttcg gtgaaatcac cattgttcac actcccacct
     6301 cccgccagta caaggacatg cggcgcttca tcggcgccca tccccacccc actggactcc
     6361 ttgtttccca attcaaagct gcccccctct atgtccgcat ctccgacaac cggatcctgg
     6421 accttgactt ccccggagtc gtcgtctgta agcaggcgta cggataccgc gccgcgacct
     6481 tcgaaggtct gtgcggttcc ccgcttgtta cggatgatcc ctccggagtc aagattctgg
     6541 gcctccacgt cgctggagtc gcgggcacga gcggattctc tgcccccatc caccccatcc
     6601 tcggccaaat cactcaattt gccactaccc aacaatctct cattgttccc actgctgaag
     6661 tccggcctgg cgtcaacgtc aaccggatgt cccgcttgca cccctctccc gcctacggtg
     6721 ccttccctgt caagaaacaa cccgctcccc tcaagcgcaa cgacaagcgc ctgcaggagg
     6781 gcgtcgatct tgacacccag ctcttcctca aacacggcaa aggcgacgtc accgagccct
     6841 ggcccggtct cgaagctgct gctgacctct acttctccac cttccccact tcccttccag
     6901 tcctcaccca ggaacaggcc atccatggaa caccaaacat ggagggcctg gacatgggcc
     6961 aggcggctgg atacccctgg aacacccttg ggcgctcccg gcgatccctc tttgacgagg
     7021 ttgaaccggg agtgttcgtc cccaaacccg aactccaagc tgaaatcaac cagacacttg
     7081 aagacccgga ctacgtgtac tccaccttcc tcaaggatga actcagacca acggctaagg
     7141 ttgaacaggg actgaccaga atcgtcgaag ctgctcccat ccatgctatc gtcgctggcc
     7201 ggatgctcct cggtggtctc atcgactaca tgcaggggcg tcccggcgag cacggtagtg
     7261 cggttggttg caacccagac gttcactgga cttccttctt ctacgccttc agcgagttct
     7321 ctcaggtata cgatctggac tacaagtgtt ttgatgctac ccttccttct gctgtcttca
     7381 cccttgttgc tgaccatctt acccgcatca ccggagatcc ccgcgtcggt cgctacatcc
     7441 actccatccg ccattcacac cacatctatg gcaaccggat gtacgacatg atcgggggga
     7501 atccctccgg ttgtgtggcc acctccatcc tcaacaccat catcaacaac atctgtgtcc
     7561 tctctgccct catccaacac cctgatttct ctccctcccg cttccacatc ctggcgtacg
     7621 gtgacgacgt catctacgct acggaacccc ccatccatcc ttccttcctt cgggagttct
     7681 accagaaaca cacccctctg gtggtcaccc ccgcaaacaa aggccaggat ttcccaccca
     7741 cgtctacgat ctatgaagtc accttcctca aacgctggtt cgttccggac gatgttagac
     7801 ccatctacat tcaccctgtg atggaccccg acacgtacga gcaatcggta atgtggttgc
     7861 gtgatggaga tttccaagat gtggtcacct ctctctgcca cctggccttc cacagcggac
     7921 ccaagaccta cgctgcctgg tgcatgaaag tgcgcgagca gtgcctcaag tccgggttcg
     7981 cccccaactt cctaccctac tcctacctcc aactccgctg gctaaatctg ctggctgcct
     8041 gatagttagg gaacacagtg cctcggcacg cttagttagt tagaaccctt tgcccactcc
     8101 tccatggtga tataaagacc acccacttcc ttcgggtgag cccctaagcc atggttgtac
     8161 tgcactatca tcctaagacg gtccttcttc ggatcgcaat ctcaccctgg tgccgcgctt
     8221 ccttcgggaa ctgcacccgc ggaccagggc cgtctttgaa cttttctaac tgttcttaca
     8281 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag cttacgcgtg gcactggccg
     8341 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8401 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8461 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     8521 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     8581 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8641 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8701 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8761 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8821 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8881 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     8941 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     9001 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9061 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     9121 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     9181 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     9241 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9301 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10081 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10261 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10561 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    10621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11161 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    11221 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11281 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    11341 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    11401 atttcacaca ggaaacagct atgaccatga ttacgaattc ggccgcctcg gcctaatacg
    11461 actcactata gggccccccc accccctttt caactgatga ggccgaaagg ccgaaaaccc
    11521 ggtatcccgg gttc
//
