pAttR-YFP-2xHA vector (V009395)

LOCUS       40924_5064        6572 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAttR-YFP-2xHA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Conn SJ.
  TITLE     Magnesium transporters in Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Conn SJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6572)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6572)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUL-2010) Agriculture, Food "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..756
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      756..898
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
                     /regulatory_class="enhancer"
     protein_bind    904..1028
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1053..1083
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1137..1793
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2138..2440
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2484..2608)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2610..3326
                     /label=EYFP
                     /note="enhanced YFP"
     CDS             3336..3362
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3372..3398
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     promoter        complement(3683..3701)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3722..3738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3746..3762)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3770..3800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3815..3836)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4124..4712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4886..5743)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5744..5848)
                     /label=AmpR promoter
     rep_origin      complement(5874..6329)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6471..6487
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6494..6512
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6537..6553
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"