LOCUS       40924_5064        6572 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAttR-YFP-2xHA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Conn SJ.
  TITLE     Magnesium transporters in Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Conn SJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6572)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6572)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUL-2010) Agriculture, Food "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..756
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      756..898
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
                     /regulatory_class="enhancer"
     protein_bind    904..1028
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1053..1083
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1137..1793
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2138..2440
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2484..2608)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2610..3326
                     /label=EYFP
                     /note="enhanced YFP"
     CDS             3336..3362
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3372..3398
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     promoter        complement(3683..3701)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3722..3738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3746..3762)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3770..3800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3815..3836)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4124..4712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4886..5743)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5744..5848)
                     /label=AmpR promoter
     rep_origin      complement(5874..6329)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6471..6487
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6494..6512
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6537..6553
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
       61 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      121 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      361 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      421 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      481 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      541 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      721 ctctatataa ggaagttcat ttcatttgga gaggacctcg agaattctca acacaacata
      781 tacaaaacaa acgaatctca agcaatcaag cattctactt ctattgcagc aatttaaatc
      841 atttctttta aagcaaaagc aattttctga aaattttcac catttacgaa cgataggcct
      901 aggacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      961 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     1021 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     1081 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     1141 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     1201 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     1261 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     1321 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     1381 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     1441 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     1501 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     1561 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     1621 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     1681 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
     1741 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
     1801 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     1861 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa
     1921 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     1981 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     2041 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     2101 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     2161 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     2221 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     2281 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     2341 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     2401 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     2461 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     2521 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     2581 ctcgttcagc tttcttgtac aaagtggtca tggtgagcaa gggcgaggag ctgttcaccg
     2641 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2701 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2761 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt
     2821 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2881 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2941 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3001 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3061 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3121 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3181 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     3241 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3301 ctctcggcat ggacgagctg tacaagagat ctatctaccc atacgatgtt ccagattacg
     3361 ctagatctat ctacccatac gatgttccag attacgctag atctatctag agtccgcaaa
     3421 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga
     3481 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     3541 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     3601 cctaaaacca aaatccagtg acctgcagcc cgggggatcc actagagcgg ccgccaccgc
     3661 ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     3721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4561 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     5881 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     5941 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     6001 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6061 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     6121 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6181 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6241 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6301 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     6361 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     6421 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     6481 ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc ccccctcgag
     6541 gtcgacggta tcgataagct tgatatcgaa tt
//
