pattP-ampicillin-attP vector (V009397)

LOCUS       40924_5054        3171 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pattP-ampicillin-attP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E.
  TITLE     PhiC31 Mediated Cassette Exchange into a BAC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2007) LBI-CR, LBI-CR, Wahringer Str. 13a, Vienna 
            A-1090, Austria
REFERENCE   3  (bases 1 to 3171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2007) LBI-CR, LBI-CR, Wahringer Str. 13a, Vienna A-1090, 
            Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          98..327
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     polyA_signal    414..637
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    672..722
                     /label=minimal attP site
                     /note="minimal attP site"
     promoter        887..991
                     /label=AmpR promoter
     CDS             992..1849
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1853..1903
                     /label=minimal attP site
                     /note="minimal attP site"
     primer_bind     complement(2011..2027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2035..2051)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2059..2089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2104..2125)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2413..3001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"