LOCUS       40924_5054        3171 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pattP-ampicillin-attP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E.
  TITLE     PhiC31 Mediated Cassette Exchange into a BAC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2007) LBI-CR, LBI-CR, Wahringer Str. 13a, Vienna 
            A-1090, Austria
REFERENCE   3  (bases 1 to 3171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2007) LBI-CR, LBI-CR, Wahringer Str. 13a, Vienna A-1090, 
            Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          98..327
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     polyA_signal    414..637
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    672..722
                     /label=minimal attP site
                     /note="minimal attP site"
     promoter        887..991
                     /label=AmpR promoter
     CDS             992..1849
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1853..1903
                     /label=minimal attP site
                     /note="minimal attP site"
     primer_bind     complement(2011..2027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2035..2051)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2059..2089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2104..2125)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2413..3001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caattggata tctggatcca cgaattcagt ggatccacta gtaacggccg ccagtgtgct
       61 ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg
      121 ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc
      181 tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt
      241 ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc
      361 atgcatctag ggcggccaat tcagcggccc tagagctcgc tgatcagcct cgactgtgcc
      421 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      481 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      541 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      601 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      661 tggggctcga ggaccctacg cccccaactg agagaactca aaggttaccc cagttggggc
      721 acggcggccg cctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
      781 ggcttaacta tgcggcatca gagcagattg tactgagagt cgaccatagg ggatcgggag
      841 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      901 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      961 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1021 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1081 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1141 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1201 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1261 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1321 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1381 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1441 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1501 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1561 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1621 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1681 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1741 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1801 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aagaccctac
     1861 gcccccaact gagagaactc aaaggttacc ccagttgggg cacttaatta acggctagct
     1921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     1981 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2041 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2101 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2161 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2221 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2281 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2401 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2461 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2521 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2581 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     2641 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2701 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2761 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2821 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2881 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2941 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3001 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3061 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3121 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca g
//
