PANTS-AraLambdaRED vector (V009493)

Basic Vector Information

      • Vector Name:
      • PANTS-AraLambdaRED
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5802 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Yoon YG, Koob MD.

PANTS-AraLambdaRED vector Vector Map

PANTS-AraLambdaRED5802 bp60012001800240030003600420048005400phage lambda ti terminator; phage lambda att sitepBR322 replication oriblaAmpR promoterAraCaraBAD promotergambetphage lambda ti terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

PANTS-AraLambdaRED vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5802 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PANTS-AraLambdaRED, complete sequence.
ACCESSION   JQ301470
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Yoon YG, Koob MD.
  TITLE     Nonreplicating Intracellular Bacterial Vector for Conjugative DNA 
            Transfer into Mitochondria
  JOURNAL   Pharm. Res. (2012) In press
  PUBMED    22350804
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Koob MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2011) Lab Medicine and Pathology, ITN and IHG, 
            University of Minnesota, 2101 6th St SE, WMBB, Minneapolis, MN 
            55455, USA
REFERENCE   3  (bases 1 to 5802)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Cloning vector PANTS-AraLambdaRED"
                     /mol_type="other DNA"
                     /db_xref="taxon:1151784"
     misc_recomb     complement(4..392)
                     /note="phage lambda ti terminator; phage lambda att site"
     protein_bind    110..341
                     /gene="attP"
                     /label=-lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     rep_origin      complement(415..1360)
                     /direction=LEFT
                     /label=pBR322 replication ori
                     /note="pBR322 replication ori"
     rep_origin      complement(660..1248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1431..2291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AFC75890.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1431..2291)
                     /gene="bla"
                     /label=bla
     promoter        complement(2292..2396)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2433..3311)
                     /codon_start=1
                     /gene="araC"
                     /product="AraC"
                     /label=araC
                     /note="repressor; induced by arabinose"
                     /protein_id="AFC75891.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     gene            complement(2433..3311)
                     /gene="araC"
                     /label=araC
     promoter        3338..3622
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3658..4074
                     /codon_start=1
                     /gene="gam"
                     /product="lambda gam recombinase"
                     /label=gam
                     /protein_id="AFC75892.1"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            3658..4074
                     /gene="gam"
                     /label=gam
     CDS             4080..4865
                     /codon_start=1
                     /gene="bet"
                     /product="lambda bet recominase"
                     /label=bet
                     /protein_id="AFC75893.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     gene            4080..4865
                     /gene="bet"
                     /label=bet
     CDS             4862..5542
                     /codon_start=1
                     /gene="exo"
                     /product="lambda exonuclease"
                     /label=exo
                     /note="exonuclease that enhances recombination"
                     /protein_id="AFC75894.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     gene            4862..5542
                     /gene="exo"
                     /label=exo
     regulatory      5532..5801
                     /regulatory_class="terminator"
                     /note="phage lambda ti terminator"
     terminator      5543..5787
                     /label=-lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"

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