LOCUS       40924_4549        5802 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PANTS-AraLambdaRED, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Yoon YG, Koob MD.
  TITLE     Nonreplicating Intracellular Bacterial Vector for Conjugative DNA 
            Transfer into Mitochondria
  JOURNAL   Pharm. Res. (2012) In press
  PUBMED    22350804
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Koob MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2011) Lab Medicine and Pathology, ITN and IHG, 
            University of Minnesota, 2101 6th St SE, WMBB, Minneapolis, MN 
            55455, USA
REFERENCE   3  (bases 1 to 5802)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5802)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pharm. Res.
            (2012) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-DEC-2011) Lab Medicine and Pathology, ITN and IHG, University of
            Minnesota, 2101 6th St SE, WMBB, Minneapolis, MN 55455, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(110..341)
                     /label=lambda attP
                     /note="integrase from phage lambda"
     rep_origin      complement(660..1248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1434..2291)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2292..2396)
                     /label=AmpR promoter
     CDS             complement(2436..3311)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3338..3622
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3658..4071
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda 
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             4080..4862
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             4862..5539
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the 
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      5543..5787
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 gctagctttg cactggattg cgaggctttg tgcttctctg gagtgcgaca ggtttgatga
       61 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      121 aataccatct aagtagttga ttcatagtga ctgcatatgt tgtgttttac agtattatgt
      181 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcagct tttttatact aagttggcat tataaaaaag cattgcttat caatttgttg
      301 caacgaacag gtcactatca gtcaaaataa aatcattatt tgatttcaat tttgtcccac
      361 tccctgcctc tgtcatcacg atactgtgat gccatggggc gcgccagcgg ccgcactagt
      421 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
      481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1081 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1321 atgagattat caaaaaggat cttcacctag atcctttttt gcggccgcaa aaattaaaaa
     1381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2401 tttccccgaa aagtgccacc tgcatcgatt tattatgaca acttgacggc tacatcattc
     2461 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
     2521 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
     2581 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
     2641 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
     2701 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
     2761 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
     2821 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
     2881 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
     2941 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     3001 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     3061 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     3121 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     3181 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     3241 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
     3301 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
     3361 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
     3421 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
     3481 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     3541 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     3601 caactctcta ctgtttctcc atacccgttt ttttgggaat tcgagctctt ataaaaaatg
     3661 gatattaata ctgaaactga gatcaagcaa aagcattcac taaccccctt tcctgttttc
     3721 ctaatcagcc cggcatttcg cgggcgatat tttcacagct atttcaggag ttcagccatg
     3781 aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca ctaccagcag
     3841 ctcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg cctgccccag
     3901 cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag caaaaaatcc
     3961 attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga acacatccgg
     4021 tacatggttg aaaccattgc tcaccaccag gttgatattg attcagaggt ataaaacgaa
     4081 tgagtactgc actcgcaacg ctggctggga agctggctga acgtgtcggc atggattctg
     4141 tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt gatgccagcg
     4201 atgcgcagtt catcgcatta ctgatcgttg ccaaccagta cggccttaat ccgtggacga
     4261 aagaaattta cgcctttcct gataagcaga atggcatcgt tccggtggtg ggcgttgatg
     4321 gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt gagcaggaca
     4381 atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc tgcgttaccg
     4441 aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga gaaatcacgg
     4501 ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt cagtgtgccc
     4561 gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc attgtcgaaa
     4621 atactgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt aacgatgaaa
     4681 ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat gacgacttat
     4741 tgccgctctg ttcccagata tttcgccgcg acattcgtgc atcgtcagaa ctgacacagg
     4801 ccgaagcagt aaaagctctt ggattcctga aacagaaagc cgcagagcag aaggtggcag
     4861 catgacaccg gacattatcc tgcagcgtac cgggatcgat gtgagagctg tcgaacaggg
     4921 ggatgatgcg tggcacaaat tacggctcgg cgtcatcacc gcttcagaag ttcacaacgt
     4981 gatagcaaaa ccccgctccg gaaagaagtg gcctgacatg aaaatgtcct acttccacac
     5041 cctgcttgct gaggtttgca ccggtgtggc tccggaagtt aacgctaaag cactggcctg
     5101 gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg gcgtgaatgt
     5161 tactgaatcc ccgatcatct atcgcgacga aagtatgcgt accgcctgct ctcccgatgg
     5221 tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc gggatttcat
     5281 gaagttccgg ctcggtggtt tcgaggccat aaagtcagct tacatggccc aggtgcagta
     5341 cagcatgtgg gtgacgcgaa aaaatgcctg gtactttgcc aactatgacc cgcgtatgaa
     5401 gcgtgaaggc ctgcattatg tcgtgattga gcgggatgaa aagtacatgg cgagttttga
     5461 cgagatcgtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt
     5521 atttggggag caatggcgat gacgcatcct cacgataata tccgggtagg cgcaatcact
     5581 ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt tatccgaaat
     5641 ccactgaaag cacagcggct ggctgaggag ataaataata aacgaggggc tgtatgcaca
     5701 aagcatcttc tgttgagtta agaacgagta tcgagatggc acatagcctt gctcaaattg
     5761 gaatcaggtt tgtgccaata ccagtagaaa cagacgaaga at
//
