pANT1201 vector (V009497)

LOCUS       40924_4524        6607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pANT1201, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Characterization of the Streptomyces sp. strain C5 snp locus and 
            development of snp-derived expression vectors
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1647-1654 (2003)
  PUBMED    12620855
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2001) School of Dentistry, Case Western Reserve 
            University, 2123 Abington Rd, Cleveland, OH 44106, USA
REFERENCE   3  (bases 1 to 6607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2003"; volume: "69"; issue: "3"; pages:
            "1647-1654"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-DEC-2001) School of Dentistry, Case Western Reserve University, 
            2123 Abington Rd, Cleveland, OH 44106, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(156..1526)
                     /codon_start=1
                     /product="replication protein pIJ101 Rep"
                     /label=replication protein pIJ101 Rep
                     /protein_id="AAL61986.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDAAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     primer_bind     2811..2827
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3015..3806)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(3848..3888)
                     /label=aprR promoter
                     /note="aprR promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4102..4118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4126..4142)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4150..4180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4195..4216)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4504..5092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5197..5327
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      complement(5327..5367)
                     /label=snpA promoter
                     /note="snpA promoter"
                     /regulatory_class="promoter"
     CDS             5585..6526
                     /codon_start=1
                     /product="LysR-like transcriptional activator SnpR"
                     /label=LysR-like transcriptional activator SnpR
                     /protein_id="AAL61988.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"