LOCUS       40924_4524        6607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pANT1201, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Characterization of the Streptomyces sp. strain C5 snp locus and 
            development of snp-derived expression vectors
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1647-1654 (2003)
  PUBMED    12620855
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2001) School of Dentistry, Case Western Reserve 
            University, 2123 Abington Rd, Cleveland, OH 44106, USA
REFERENCE   3  (bases 1 to 6607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2003"; volume: "69"; issue: "3"; pages:
            "1647-1654"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-DEC-2001) School of Dentistry, Case Western Reserve University, 
            2123 Abington Rd, Cleveland, OH 44106, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(156..1526)
                     /codon_start=1
                     /product="replication protein pIJ101 Rep"
                     /label=replication protein pIJ101 Rep
                     /protein_id="AAL61986.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDAAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     primer_bind     2811..2827
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3015..3806)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(3848..3888)
                     /label=aprR promoter
                     /note="aprR promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4102..4118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4126..4142)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4150..4180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4195..4216)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4504..5092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5197..5327
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      complement(5327..5367)
                     /label=snpA promoter
                     /note="snpA promoter"
                     /regulatory_class="promoter"
     CDS             5585..6526
                     /codon_start=1
                     /product="LysR-like transcriptional activator SnpR"
                     /label=LysR-like transcriptional activator SnpR
                     /protein_id="AAL61988.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"
ORIGIN
        1 tcggcggtac cgcctgcacg aagtgccggc ggggccgacc ccgggcgagt aatcccagga
       61 ttactcccgc ggcttcgacc ccggccgccg tcgccgcgta cgtcaccgac ccccgccgta
      121 cgtcaccggg atgacgtacg gcggggggga gcgagttagt gcgaagtggg cccacttgcg
      181 agccgggcga tgtgccgggc ggcccgctcc tggcggtcgt cggcgtcgtc gtcctggtcg
      241 tcgtcctgct ctcgccgtcg gcgtgcagtt gcttcctcgc ggcgctgggc gagggcggcg
      301 agcatgtcgg cgtacgcctc ggccacctcc cccgccgtga gcaccaccac tgtgtcggcc
      361 gcgtcggcca gcgccaggac ctcccgcacc cgttcgccca cggccgccgc atcctcgttg
      421 ccgtccttgc cttcggcggc ccgggtcgcc tcgaggtcga gggcgcggcg ggtgaccgcg
      481 tgccatccgt cctcggtcac ggcgaccccg gcccgcagct ccccgccgtc ggcgtcggcc
      541 gccaggagca gatcgaggtc gtcggcctcg gtgtcgccgc cgtcgagccc gagcatctgc
      601 cgcaggtagc gggtccattc gatggcccgg cgtccccggg ttgcccgctc gtactcgtgc
      661 cagcgcgaga ggttccactc cagcgagccg accccggcgg cgtcgtcctc ggtcatgccg
      721 ccggtcaggt ccccgatccg tccgaggagt tcgaacgggg cgacgttccc gccggtcgcc
      781 gtcttgaggt cggcgcgggc gagttcgagg gcgggcgcct tcccgtcctg ggtcttggcg
      841 atgtactcgg cgaggtcgtt ggcgtcgcgc tcggtctcca gccgcttgaa gtcgacgccg
      901 tgccggtcgt cgggcgtgaa ggcggggttg accttgcgca gggcggcggt ccacacggac
      961 cgccagtgcc cctgccactc gtcgagcgcg gcgccggtcg gctcgaaggt ggcgacgatc
     1021 tgcttcgcgg accgctcccc ctcggtccgg ccgccgacca ggacgatcgc gtggatgtgc
     1081 gggtgccagc cgttgatctg ccccacggtg acttcggtcg cgcggatcat gccgacgtac
     1141 ccgatccggt ctcggatgcc ctcgcggtcg gcggcccggt gcccgtcctt ggcccggcgt
     1201 ccggcccacg tgccgcccgt gatcagtcgc tggtaggcgc ccggccgccg ggggctgtcc
     1261 ggcgtcttcc gggtgccctg gagggcgtcc atgaggtccg cgagccggtc cgtgtgccca
     1321 tggcgggccg tgaaggtgac caggtaggcg gtccccccgc gcttgatcca ctcgaccacg
     1381 gcggcggtga tctcctcggc ccgcttgtgc cggatcgtgg cggcgcagac cgggcagagc
     1441 cagatccgcc cgcaccgcat caggcccagg accacggacg ttccggccgc cgtctgggcg
     1501 acgatcacgc cggaggcagg gtccatcagg gcgcggccgc agcccttgca cgcggcgtcc
     1561 ccgctgatcc gccacagcgt ccggcggcgg ctgtaccggg cggctttccg cagtcgggca
     1621 gcctcggtcc gcgacgtgct tcctacttcc cagaggctgt cgcctctcgg gctctcccca
     1681 tccaccccgt ccggagaaac cgcaggtcgg aggggtgcgg gaaactctgt tgtttctttc
     1741 ccaaggtgtt cgcttttgcc tcgggcggca tctcgcgtca cacgcgcgat cgcccgcttc
     1801 gctgccatcc ggcagcggtc tgagcagtag atacgcggcc gtttgcccgg tgtgtgggca
     1861 attgcggtcc cgcagtggca gcggggcccg gcgggccgat ctggcaatgc ctcggcatcg
     1921 ctccgtactc tgggcacgag caacgttcct gtctcgcccg gctaaggggc gcgagtctgg
     1981 gagcggacgg gtcggaggtg cgaagtccgg cccgttgctc tttggtctgg tgggaatcct
     2041 ggcaccaatc gggccagagg ttccctccgc cactcccgac gccccttggg gctggtgtga
     2101 cttggagggc cgaagagagc cccgccgggt atccggcggg gctttgacgt gcggtcagtg
     2161 cgtgtgtcgg cgagcgatgg ccacgaggcc ctggaagccg agcggtccgg cgaagtcggc
     2221 ccagtcgcaa ccgggctcag cgcagtgggc ggaccagcca ccgccgttgg ggtcctggac
     2281 caggttcacg gtcccctcgg tcaggcgtcc gtcgaagtcg gtcatggtcg gtctcctggt
     2341 gggtgggggc ggggcgccag cacgaagtgc cggcgccccg cgggggttgg tcgggtcagg
     2401 cgccgaaccg gcgggcggcg gcggcgacca ggccgtcggc ggcggccatg gcgcggtcgc
     2461 ggtcggtggt gagggcggtg cggtcggcgg cggcccagtc ctgctctcac gcaacgtcta
     2521 cgtggacgct cagggcgaca cgatcgaggt cggggagtcc gtcgctccgg cgggccgctg
     2581 gcgcgtccac cgggactgat caaggcgaat acttcatatg cggtgtgaaa taccgcacag
     2641 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     2701 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     2761 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     2821 ggccagtgaa ttaattcggg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     2881 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     2941 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     3001 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     3061 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     3121 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     3181 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     3241 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     3301 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     3361 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     3421 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     3481 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     3541 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     3601 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     3661 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3721 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3781 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatccccc
     3841 ggcttttcgc cattcgtatt gcacgacatt gcactccacc gctgatgaca tcagtcgatc
     3901 atagcacgat caacggcact gttgcaaata gtcggtggtg ataaacttat catccccttt
     3961 tgctgatgga gctgcacatg aacccattca aaggccggca ttttcagcgt gacatcattc
     4021 tgtgggccgt acgctggtac tgcaaatacg gcatcagtta ccgtgagctg caggcatgca
     4081 agctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4921 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaagctcc atggacccgt
     5221 gcaggggccc cgacacggtc ggggcccctg cactctatct attcacgcgt ggtaccacta
     5281 gtaagcttgg atcctgcaga gctctagatc tgaattcgca tgcgcatcga tgactcctcg
     5341 aggtgggggg atgagtcaga catcgtgagt gtccgtgccc gtacggccgt tgtgatgatg
     5401 gcaaccgccg atagggccgc cctatccccc tccgtcccca cctgccccgc cctgcccggc
     5461 ggccccggcc ggggcccctc ccgatccggg ccgacgcctc gcgccgtgaa ggcccgatgg
     5521 tcctgatcgg tcgccatttt gtatgtctgg tgaaagctga acttccgcct taccctccca
     5581 ggacatggag cttgaggtca ggcacctcag ggcgctgtgc gccatcgccg acaccggcag
     5641 cctgcaccgc gcggcacgcc aactgggagt gacacagccc tcgttgagca cgcagctgcg
     5701 gcgcatcgaa cacgagctgg gcggtgccct gttcgtccgg gcccgcaccg gctgccgccc
     5761 cacaccgctg ggccggctgg tgctcagtcg tgcccgcccc ctggtggccg aattgcgctc
     5821 cctcgtcagc gaggcccgcg ccgccgccgt cggcggacgc cagctgcgcg tcggctccac
     5881 ggccagccgg gccctggcgg gctggctgcg ccggctccgc cggcactggc aggaacccac
     5941 cctgcacatg gacgtctccg ccaacgccct gctgcgcatg gtggccgacg gccacctcga
     6001 cgtcgccttc gtgcacgagg tcgagggcag cccgctgcgc gtccccgaag ggctccgggt
     6061 ccgcgtactg gtccagcggg aaccgcagtt cgtctgcctg ccggccgacc acccggccgc
     6121 ggcgaagccg tcgtacgcct cgccgacctg gcccacgacc cgctggatga tcgaccccac
     6181 cgtcgacggc gagtgggacg cggtgcgccg ggtcctgcgc gccgagggac tcgacccgcg
     6241 catcctgcac ggggactacc acaccgccgc gtccctggtc gccaccggcg aggtcgtcac
     6301 cgtggtccag ccgacctcgc cctcccgcgc cgagacggcc gtccgccggc tgcacggcga
     6361 cccgctcggc gtacggctgc tgctggcggc ccgcacggac acggaactgg agggcgtcta
     6421 ccccgacctc gcggaggcct acggggaggt cgcccggcag gccccggcgt accgggagtg
     6481 gctggaacgc agtgggtcgg gggcactcgt cccagccctc ccgtgacgac cgcgtacccc
     6541 ggcccccttc ccgtaacccg acgggagatg ccctgggggg ggacggcggt gcgggggatg
     6601 cagcggg
//
