pANT1200 vector (V009498)

LOCUS       V009498                 8058 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009498
VERSION     V009498
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Characterization of the Streptomyces sp. strain C5 snp locus and
            development of snp-derived expression vectors
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1647-1654 (2003)
   PUBMED   12620855
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2001) School of Dentistry, Case Western Reserve
            University, 2123 Abington Rd, Cleveland, OH 44106, USA
REFERENCE   3  (bases 1 to 8058)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2003"; volume: "69"; issue: "3"; pages:
            "1647-1654"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-DEC-2001) School of Dentistry, Case Western Reserve University,
            2123 Abington Rd, Cleveland, OH 44106, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        407..511
                     /label="AmpR promoter"
     CDS             512..1369
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1543..2131
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      2134..2265
                     /label="ColE1"
                     /note="ColE1"
     protein_bind    2419..2440
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2455..2485
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2493..2509
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2517..2533
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2690..4060)
                     /codon_start=1
                     /product="replication protein pIJ101 Rep"
                     /label="replication protein pIJ101 Rep"
                     /protein_id="AAL61983.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDPAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     CDS             5326..6132
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     misc_feature    6562..6672
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      complement(6692..6732)
                     /label="snpA promoter"
                     /note="snpA promoter"
                     /regulatory_class="promoter"
     CDS             6950..7891
                     /codon_start=1
                     /product="LysR-like transcriptional activator SnpR"
                     /label="LysR-like transcriptional activator SnpR"
                     /protein_id="AAL61985.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"