LOCUS       V009498                 8058 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009498
VERSION     V009498
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Characterization of the Streptomyces sp. strain C5 snp locus and
            development of snp-derived expression vectors
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1647-1654 (2003)
   PUBMED   12620855
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2001) School of Dentistry, Case Western Reserve
            University, 2123 Abington Rd, Cleveland, OH 44106, USA
REFERENCE   3  (bases 1 to 8058)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2003"; volume: "69"; issue: "3"; pages:
            "1647-1654"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-DEC-2001) School of Dentistry, Case Western Reserve University,
            2123 Abington Rd, Cleveland, OH 44106, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        407..511
                     /label="AmpR promoter"
     CDS             512..1369
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1543..2131
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      2134..2265
                     /label="ColE1"
                     /note="ColE1"
     protein_bind    2419..2440
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2455..2485
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2493..2509
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2517..2533
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2690..4060)
                     /codon_start=1
                     /product="replication protein pIJ101 Rep"
                     /label="replication protein pIJ101 Rep"
                     /protein_id="AAL61983.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDPAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     CDS             5326..6132
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     misc_feature    6562..6672
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      complement(6692..6732)
                     /label="snpA promoter"
                     /note="snpA promoter"
                     /regulatory_class="promoter"
     CDS             6950..7891
                     /codon_start=1
                     /product="LysR-like transcriptional activator SnpR"
                     /label="LysR-like transcriptional activator SnpR"
                     /protein_id="AAL61985.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"
ORIGIN
        1 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
       61 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      121 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      181 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      241 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      301 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      361 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      421 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      481 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      541 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      601 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      661 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      721 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      781 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      841 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      901 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      961 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1021 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1081 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1141 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1201 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1261 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1321 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1381 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1441 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1501 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1561 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1621 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1681 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1741 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1801 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1861 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1921 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1981 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2041 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2101 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2161 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2221 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2281 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     2341 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     2401 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     2461 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2521 aaacagctat gaccatgatt acgccgcctg cacgaagtgc cggcggggcc gaccccgggc
     2581 gagtaatccc aggattactc ccgcggcttc gaccccggcc gccgtcgccg cgtacgtcac
     2641 cgacccccgc cgtacgtcac cgggatgacg tacggcgggg gggagcgagt tagtgcgaag
     2701 tgggcccact tgcgagccgg gcgatgtgcc gggcggcccg ctcctggcgg tcgtcggcgt
     2761 cgtcgtcctg gtcgtcgtcc tgctctcgcc gtcggcgtgc agttgcttcc tcgcggcgct
     2821 gggcgagggc ggcgagcatg tcggcgtacg cctcggccac ctcccccgcc gtgagcacca
     2881 ccactgtgtc ggccgcgtcg gccagcgcca ggacctcccg cacccgttcg cccacggccg
     2941 ccggatcctc gttgccgtcc ttgccttcgg cggcccgggt cgcctcgagg tcgagggcgc
     3001 ggcgggtgac cgcgtgccat ccgtcctcgg tcacggcgac cccggcccgc agctccccgc
     3061 cgtcggcgtc ggccgccagg agcagatcga ggtcgtcggc ctcggtgtcg ccgccgtcga
     3121 gcccgagcat ctgccgcagg tagcgggtcc attcgatggc ccggcgtccc cgggttgccc
     3181 gctcgtactc gtgccagcgc gagaggttcc actccagcga gccgaccccg gcggcgtcgt
     3241 cctcggtcat gccgccggtc aggtccccga tccgtccgag gagttcgaac ggggcgacgt
     3301 tcccgccggt cgccgtcttg aggtcggcgc gggcgagttc gagggcgggc gccttcccgt
     3361 cctgggtctt ggcgatgtac tcggcgaggt cgttggcgtc gcgctcggtc tccagccgct
     3421 tgaagtcgac gccgtgccgg tcgtcgggcg tgaaggcggg gttgaccttg cgcagggcgg
     3481 cggtccacac ggaccgccag tgcccctgcc actcgtcgag cgcggcgccg gtcggctcga
     3541 aggtggcgac gatctgcttc gcggaccgct ccccctcggt ccggccgccg accaggacga
     3601 tcgcgtggat gtgcgggtgc cagccgttga tctgccccac ggtgacttcg gtcgcgcgga
     3661 tcatgccgac gtacccgatc cggtctcgga tgccctcgcg gtcggcggcc cggtgcccgt
     3721 ccttggcccg gcgtccggcc cacgtgccgc ccgtgatcag tcgctggtag gcgcccggcc
     3781 gccgggggct gtccggcgtc ttccgggtgc cctggagggc gtccatgagg tccgcgagcc
     3841 ggtccgtgtg cccatggcgg gccgtgaagg tgaccaggta ggcggtcccc ccgcgcttga
     3901 tccactcgac cacggcggcg gtgatctcct cggcccgctt gtgccggatc gtggcggcgc
     3961 agaccgggca gagccagatc cgcccgcacc gcatcaggcc caggaccacg gacgttccgg
     4021 ccgccgtctg ggcgacgatc acgccggagg cagggtccat cagggcgcgg ccgcagccct
     4081 tgcacgcggc gtccccgctg atccgccaca gcgtccggcg gcggctgtac cgggcggctt
     4141 tccgcagtcg ggcagcctcg gtccgcgacg tgcttcctac ttcccagagg ctgtcgcctc
     4201 tcgggctctc cccatccacc ccgtccggag aaaccgcagg tcggaggggt gcgggaaact
     4261 ctgttgtttc tttcccaagg tgttcgcttt tgcctcgggc ggcatctcgc gtcacacgcg
     4321 cgatcgcccg cttcgctgcc atccggcagc ggtctgagca gtagatacgc ggccgtttgc
     4381 ccggtgtgtg ggcaattgcg gtcccgcagt ggcagcgggg cccggcgggc cgatctggca
     4441 atgcctcggc atcgctccgt actctgggca cgagcaacgt tcctgtctcg cccggctaag
     4501 gggcgcgagt ctgggagcgg acgggtcgga ggtgcgaagt ccggcccgtt gctctttggt
     4561 ctggtgggaa tcctggcacc aatcgggcca gaggttccct ccgccactcc cgacgcccct
     4621 tggggctggt gtgacttgga gggccgaaga gagccccgcc gggtatccgg cggggctttg
     4681 acgtgcggtc agtgcgtgtg tcggcgagcg atggccacga ggccctggaa gccgagcggt
     4741 ccggcgaagt cggcccagtc gcaaccgggc tcagcgcagt gggcggacca gccaccgccg
     4801 ttggggtcct ggaccaggtt cacggtcccc tcggtcaggc gtccgtcgaa gtcggtcatg
     4861 gtcggtctcc tggtgggtgg gggcggggcg ccagcacgaa gtgccggcgc cccgcggggg
     4921 ttggtcgggt caggcgccga accggcgggc ggcggcggcg accaggccgt cggcggcggc
     4981 catggcgcgg tcgcggtcgg tggtgagggc ggtgcggtcg gcggcggccc agtcctgctc
     5041 tcacgcaacg tctacgtgga cgctcagggc gacacgatcg aggtcgggga gtccgtcgct
     5101 ccggcgggcc gctggcgcgt ccaccgggac tgatcaaggc gaatacttca tatgcgggga
     5161 tcgaccgcgc gggtcccgga cggggaagag cggggagctt tgccagagag cgacgacttc
     5221 cccttgcgtt ggtgattgcc ggtcagggca gccatccgcc atcgtcgcgt agggtgtcac
     5281 accccaggaa tcgcgtcact gaacacagca gccggtagga cgaccatgac tgagttggac
     5341 accatcgcaa atccgtccga tcccgcggtg cagcggatca tcgatgtcac caagccgtcg
     5401 cgatccaaca taaagacaac gttgatcgag gacgtcgagc ccctcatgca cagcatcgcg
     5461 gccggggtgg agttcatcga ggtctacggc agcgacagca gtccttttcc atctgagttg
     5521 ctggatctgt gcgggcggca gaacataccg gtccgcctca tcgactcctc gatcgtcaac
     5581 cagttgttca agggggagcg gaaggccaag acattcggca tcgcccgcgt ccctcgcccg
     5641 gccaggttcg gcgatatcgc gagccggcgt ggggacgtcg tcgttctcga cggggtgaag
     5701 atcgtcggga acatcggcgc gatagtacgc acgtcgctcg cgctcggagc gtcggggatc
     5761 atcctggtcg acagtgacat caccagcatc gcggaccggc gtctccaaag ggccagccga
     5821 ggttacgtct tctcccttcc cgtcgttctc tccggtcgcg aggaggccat cgccttcatt
     5881 cgggacagcg gtatgcagct gatgacgctc aaggcggatg gcgacatttc cgtgaaggaa
     5941 ctcggggaca atccggatcg gctggccttg ctgttcggca gcgaaaaggg tgggccttcc
     6001 gacctgttcg aggaggcgtc ttccgcctcg gtttccatcc ccatgatgag ccagaccgag
     6061 tctctcaacg tttccgtttc cctcggaatc gcgctgcacg agaggatcga caggaatctc
     6121 gcggccaacc gataagcgcc tctgttcctc ggacgctcgg ttcctcgacc tcgattcgtc
     6181 agtgatgatc agacgtcgaa ggtgtagtgc cgggtgtggt ccagcagggc cgccggggtg
     6241 gtgtcgttcc acggcttcat cgtctcgttg aggtcgacga cgttcggcgt gccgccgccc
     6301 ggcagatacg gggacgaggg gtgccgccgc tgccactcgg cccacagctt gtcgatgtag
     6361 gcgtggtgca gccagaacac cgggtcgttg ggggagaccc cggtcgccat ctggccgccg
     6421 acccagacat gcacccggtt gtgcagattg accccgcgcc acccttcgag atggttgcgg
     6481 aagccgtcgg agccgctgtt ccagggcgcc atgtcgtacg tcgccatcgc cagcaccgag
     6541 tcgacctcgg cacgcgttaa ctagtttaaa gctccatgga cccgtgcagg ggccccgaca
     6601 cggtcggggc ccctgcactc tatctattca cgcgtggtac cactagtaag cttggatcct
     6661 gcagagctct agatctgaat tcgcatgcgc atcgatgact cctcgaggtg gggggatgag
     6721 tcagacatcg tgagtgtccg tgcccgtacg gccgttgtga tgatggcaac cgccgatagg
     6781 gccgccctat ccccctccgt ccccacctgc cccgccctgc ccggcggccc cggccggggc
     6841 ccctcccgat ccgggccgac gcctcgcgcc gtgaaggccc gatggtcctg atcggtcgcc
     6901 attttgtatg tctggtgaaa gctgaacttc cgccttaccc tcccaggaca tggagcttga
     6961 ggtcaggcac ctcagggcgc tgtgcgccat cgccgacacc ggcagcctgc accgcgcggc
     7021 acgccaactg ggagtgacac agccctcgtt gagcacgcag ctgcggcgca tcgaacacga
     7081 gctgggcggt gccctgttcg tccgggcccg caccggctgc cgccccacac cgctgggccg
     7141 gctggtgctc agtcgtgccc gccccctggt ggccgaattg cgctccctcg tcagcgaggc
     7201 ccgcgccgcc gccgtcggcg gacgccagct gcgcgtcggc tccacggcca gccgggccct
     7261 ggcgggctgg ctgcgccggc tccgccggca ctggcaggaa cccaccctgc acatggacgt
     7321 ctccgccaac gccctgctgc gcatggtggc cgacggccac ctcgacgtcg ccttcgtgca
     7381 cgaggtcgag ggcagcccgc tgcgcgtccc cgaagggctc cgggtccgcg tactggtcca
     7441 gcgggaaccg cagttcgtct gcctgccggc cgaccacccg gccgcggcga agccgtcgta
     7501 cgcctcgccg acctggccca cgacccgctg gatgatcgac cccaccgtcg acggcgagtg
     7561 ggacgcggtg cgccgggtcc tgcgcgccga gggactcgac ccgcgcatcc tgcacgggga
     7621 ctaccacacc gccgcgtccc tggtcgccac cggcgaggtc gtcaccgtgg tccagccgac
     7681 ctcgccctcc cgcgccgaga cggccgtccg ccggctgcac ggcgacccgc tcggcgtacg
     7741 gctgctgctg gcggcccgca cggacacgga actggagggc gtctaccccg acctcgcgga
     7801 ggcctacggg gaggtcgccc ggcaggcccc ggcgtaccgg gagtggctgg aacgcagtgg
     7861 gtcgggggca ctcgtcccag ccctcccgtg acgaccgcgt accccggccc ccttcccgta
     7921 acccgacggg agatgccctg gggggggacg gcggtgcggg ggatgcagcg ggtcggcggc
     7981 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8041 agcacatccc cctttcgc
//
