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pAmin81 vector (V009505)

Basic Vector Information

pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost.

Vector Name:
pAmin81
Antibiotic Resistance:
Kanamycin
Length:
3654 bp
Type:
Cloning vector
Replication origin:
pSC101 ori
Source/Author:
Hajimorad M, Gray PR, Keasling JD.
Copy Number:
Low Copy
Growth Temperature:
30℃

pAmin81 vector Map

Copy Sequence View Map
pAmin813654 bp60012001800240030003600NeoR/KanRlambda t0 terminatorKS primerrrnB T1 terminatorRep101pSC101 oriregulatory

pAmin81 vector Sequence

LOCUS       40924_4464        3654 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAmin81, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Hajimorad M, Gray PR, Keasling JD.
  TITLE     A framework and model system to investigate linear system behavior 
            in Escherichia coli
  JOURNAL   J Biol Eng 5 (1) (2011) In press
  PUBMED    21510907
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Hajimorad M, Gray PR, Keasling JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2010) Electrical Engineering and Computer 
            Sciences, University of California, Berkeley, 231 Cory Hall, 
            Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 3654)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Eng 
            5 (1) (2011) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-2010) Electrical Engineering and Computer Sciences, 
            University of California, Berkeley, 231 Cory Hall, Berkeley, CA 
            94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             198..989
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1023..1117
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     1141..1157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1185..1271)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1840..2787)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2835..3057)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     regulatory      3549..3654
                     /regulatory_class="terminator"
     terminator      3554..3648
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"

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