LOCUS       40924_4464        3654 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAmin81, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Hajimorad M, Gray PR, Keasling JD.
  TITLE     A framework and model system to investigate linear system behavior 
            in Escherichia coli
  JOURNAL   J Biol Eng 5 (1) (2011) In press
  PUBMED    21510907
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Hajimorad M, Gray PR, Keasling JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2010) Electrical Engineering and Computer 
            Sciences, University of California, Berkeley, 231 Cory Hall, 
            Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 3654)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Eng 
            5 (1) (2011) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-2010) Electrical Engineering and Computer Sciences, 
            University of California, Berkeley, 231 Cory Hall, Berkeley, CA 
            94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             198..989
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1023..1117
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     1141..1157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1185..1271)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1840..2787)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2835..3057)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     regulatory      3549..3654
                     /regulatory_class="terminator"
     terminator      3554..3648
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttacc ggttgggtac
       61 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
      121 tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag
      181 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
      241 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      301 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      361 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      421 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      481 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
      541 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
      601 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
      661 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
      721 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
      781 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
      841 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
      901 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
      961 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg agagctcgct
     1021 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     1081 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcgctagcg ggccccccct
     1141 cgaggtcgac ggtatcgata agcttagatc ttctagggcg gcggatttgt cctactcagg
     1201 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     1261 cgttttattt gatgcctcta gcacgcgtac catgggatcc cccgggctgc aggaattcgt
     1321 acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc
     1381 agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat
     1441 tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc
     1501 agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg
     1561 tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt
     1621 acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca
     1681 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
     1741 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
     1801 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     1861 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     1921 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     1981 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
     2041 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     2101 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     2161 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     2221 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     2281 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     2341 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     2401 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     2461 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     2521 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     2581 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     2641 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     2701 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     2761 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     2821 ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt gagttgtggg
     2881 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     2941 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     3001 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     3061 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     3121 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     3181 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     3241 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     3301 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     3361 ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc
     3421 tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg
     3481 gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcccta
     3541 gggagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     3601 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagc
//
