pAKT7 vector (V009533)

LOCUS       V009533                 8492 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009533
VERSION     V009533
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Reassessing the Potential Activities of Plant CGI-58 Protein
  JOURNAL   PLoS ONE 11 (1), E0145806 (2016)
   PUBMED   26745266
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2015) Organization and Dynamics of Biological
            Membranes, Insitut de Chimie et de Biochimie Moleculaires et
            Supramoleculaires, 43 bd du 11 Novembre 1918, Villeurbanne 69622,
            France
REFERENCE   3  (bases 1 to 8492)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "1"; pages: "E0145806"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-OCT-2015) Organization and Dynamics of Biological Membranes,
            Insitut de Chimie et de Biochimie Moleculaires et Supramoleculaires,
            43 bd du 11 Novembre 1918, Villeurbanne 69622, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      783..1328
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2257..4905)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(5212..5385)
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    complement(5405..5421)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5429..5459)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(5474..5495)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5511..6590)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(6591..6668)
                     /label="lacI promoter"
     promoter        8145..8249
                     /label="AmpR promoter"
     CDS             join(8250..8492,1..615)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    8434..8492
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"