LOCUS       V009533                 8492 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009533
VERSION     V009533
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Reassessing the Potential Activities of Plant CGI-58 Protein
  JOURNAL   PLoS ONE 11 (1), E0145806 (2016)
   PUBMED   26745266
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2015) Organization and Dynamics of Biological
            Membranes, Insitut de Chimie et de Biochimie Moleculaires et
            Supramoleculaires, 43 bd du 11 Novembre 1918, Villeurbanne 69622,
            France
REFERENCE   3  (bases 1 to 8492)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "1"; pages: "E0145806"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-OCT-2015) Organization and Dynamics of Biological Membranes,
            Insitut de Chimie et de Biochimie Moleculaires et Supramoleculaires,
            43 bd du 11 Novembre 1918, Villeurbanne 69622, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      783..1328
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2257..4905)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(5212..5385)
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    complement(5405..5421)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5429..5459)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(5474..5495)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5511..6590)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(6591..6668)
                     /label="lacI promoter"
     promoter        8145..8249
                     /label="AmpR promoter"
     CDS             join(8250..8492,1..615)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    8434..8492
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
ORIGIN
        1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
       61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
      781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
      841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
      901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
      961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
     1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
     1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
     1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
     1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
     1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
     1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
     1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1921 ttatgagcca tattcaacgg gaaacgtctt gctcgacgct ctcccttatg cgactcctgc
     1981 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     2041 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     2101 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     2161 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     2221 gcgtagagga tccggagtcg tattgatttg gcgttacgcg aacgcgaagt ccgactctaa
     2281 gatgtcacgg aggttcaagt tacctttagc cggaagtgct ggcattttgt ccaattgaga
     2341 ctcgtgcaac tggtcagcga actggtcgta gaaatcagcc agtacatcac aagactcata
     2401 tgtgtcaacc atagtttcgc gcactgcttt gaacaggttc gcagcgtcag ccggaatggt
     2461 accgaaggag tcgtgaatca gtgcaaaaga ttcgattccg tacttctcgt gtgcccacac
     2521 tacagtctta cgaaggtggc taccgtcttg gctgtgtaca aagttaggag cgataccaga
     2581 ctcctgtttg tgtgcatcaa tctcgctatc tttgttggtg ttaatggtag gctgtaagcg
     2641 gaactgaccg aggaacatca ggttcaagcg cgtctgaata ggcttcttgt attcctgcca
     2701 cacagggaaa ccatcaggag ttacccaatg cacagcgcaa cgcttgcgaa gaatctctcc
     2761 agtcttctta tctttgacct cagcagccag cagcttagca gcagacttaa gccagttcat
     2821 tgcttcaacc gcagctacca ccgtcacgct cacagattcc caaatcagct tagccatgta
     2881 tccagcagcc tgattcggct gagtgaacat cggacccttg ccggaatcaa tagctggctg
     2941 aatggtatct tccagcactt gttgacggaa gccgaactct ttggacccgt aagccagcgt
     3001 catgactgaa cgcttagtca cactgcgagt aacaccgtga gccagccatt gaccagccag
     3061 tgccttagtg cccagcttga ctttctcaga gatttcacca gtgttctcat cggtcacggt
     3121 aactacttcg ttatcggtcc cattgattgc gtctgcttgt agaatctcgt tgactttctt
     3181 agcaacaatc ccgtagatgt cctgaacggt ctcactagga agcaagttaa ccgcgcgacc
     3241 acctacctca tctcggagca tcgcggagaa gtgctggatg ccagagcaag acccgtcaaa
     3301 cgccagcgga agggagcagt tatagctcag gccgtggtgc tgtaccccag cgtactcaaa
     3361 gcagaacgca aggaagcaga acggagaatc ttgctcagcc caccaagtgt tctccagtgg
     3421 agacttagcg caagccatga tgttctcgtg gttttcctca atgaacttga tgcgctcagg
     3481 gaacggaacc ttatcgacac ccgcacagtt tgcaccgtgg attttcagcc agtagtaacc
     3541 ttccttaccg attggtttac ctttcgccag cgtaagcagt cctttggtca tatcgttacc
     3601 ttgcgggttg aacattgaca cggcgtaaac acgaccgcgc cagtccatgt tgtaagggaa
     3661 ccagatggcc ttatggttag caaacttatt ggcttgctca agcatgaact caaggctgat
     3721 acggcgagac ttgcgagccc tgtccttgcg gtacacagca gcggcagcac gtttccacgc
     3781 ggtgagagcc tcaggattca tgtcgatgtc ttccggtttc atcgggagtt cttcacgctc
     3841 aatcgcaggg atgtcctcga ccggacaatg cttccacttg gtgattacgt tggcgaccgc
     3901 taggactttc ttgttgattt tccatgcggt gttttgcgca atgttaatcg ctttgtacac
     3961 ctcaggcatg taaacgtctt cgtagcgcat cagtgctttc ttactgtgag tacgcaccag
     4021 cgccagagga cgacgaccgt tagcccaata gccaccacca gtaatgccag tccacggctt
     4081 aggaggaact acgcaaggtt ggaacatcgg agagatgcca gccagcgcac ctgcacgggt
     4141 tgcgatagcc tcagcgtatt caggtgcgag ttcgatagtc tcagagtctt gacctactac
     4201 gccagcattt tggcggtgta agctaaccat tccggttgac tcaatgagca tctcgatgca
     4261 gcgtactcct acatgaatag agtcttcctt atgccacgaa gaccacgcct cgccaccgag
     4321 tagaccctta gagagcatgt cagcctcgac aacttgcata aatgctttct tgtagacgtg
     4381 ccctacgcgc ttgttgagtt gttcctcaac gtttttcttg aagtgcttag cttcaaggtc
     4441 acggatacga ccgaagcgag cctcgtcctc aatggcccga ccgattgcgc ttgctacagc
     4501 ctgaacggtt gtattgtcag cactggttag gcaagccaga gtggtcttaa tggtgatgta
     4561 cgctacggct tccggcttga tttcttgcag gaactggaag gctgtcgggc gcttgccgcg
     4621 cttagctttc acttcctcaa accagtcgtt gatgcgtgca atcatcttag ggagtagggt
     4681 agtgatgaga ggcttggcgg cagcgttatc cgcaacctca ccagctttaa gttgacgctc
     4741 aaacatcttg cggaagcgtg cttcacccat ctcgtaagac tcatgctcaa gggccaactg
     4801 ttcgcgagct aaacgctcac cgtaatggtc agccagagtg ttgaacggga tagcagccag
     4861 ttcgatgtca gagaagtcgt tcttagcgat gttaatcgtg ttcatttagt gcctcttcca
     4921 gttagtaaat ccggatcaga tcccgacgcc atcaaaaata attcgcgtct ggccttcctg
     4981 tagccagctt tcatcaacat taaatgtgag cgagtaacaa cccgtcggat tctccgtggg
     5041 aacaaacggc ggattgaccg taatgggata ggtcacgttg gtgtagatgg gcgcatcgta
     5101 accgtgcatc tgccagtttg aggggacgac gacagtatcg gcctcaggaa gatcgcactc
     5161 cagccagctt tccggcaccg cttctggtgc cggaaaccag gcaaagcgcc attcgccatt
     5221 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     5281 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     5341 acgacgttgt aaaacgacgg ccagtgaatc cgtaatcatg gtcatagctg tttcctgtgt
     5401 gaaattgtta tccgctcaca attccacaca ttatacgagc cggaagcata aagtgtaaag
     5461 cctggggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     5521 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5581 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     5641 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     5701 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     5761 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     5821 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     5881 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     5941 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     6001 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     6061 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     6121 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     6181 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     6241 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     6301 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     6361 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     6421 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     6481 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     6541 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     6601 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     6661 atggtgtccg ggatctggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
     6721 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
     6781 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
     6841 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
     6901 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
     6961 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
     7021 taaagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     7081 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     7141 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     7201 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     7261 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     7321 agagcattac gctgacttga cgggacggcg gctttgttga ataaatcgaa cttttgctga
     7381 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
     7441 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
     7501 tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg
     7561 aggcagacct cagcgctcaa agatgcaggg gtaaaagcta accgcatctt taccgacaag
     7621 gcatccggca gttcaacaga tcgggaaggg ctggatttgc tgaggatgaa ggtggaggaa
     7681 ggtgatgtca ttctggtgaa gaagctcgac cgtcttggcc gcgacaccgc cgacatgatc
     7741 caactgataa aagagtttga tgctcagggt gtagcggttc ggtttattga cgacgggatc
     7801 agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc tgtcggctgt ggcacaggct
     7861 gaacgccgga ggatcctaga gcgcacgaat gagggccgac aggaagcaaa gctgaaagga
     7921 atcaaatttg gccgcaggcg taccgtggac aggaacgtcg tgctgacgct tcatcagaag
     7981 ggcactggtg caacggaaat tgctcatcag ctcagtattg cccgctccac ggtttataaa
     8041 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8101 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8161 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8221 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8281 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8341 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8401 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8461 aagttctgct atgtggcgcg gtattatccc gt
//
