pAJM.969 vector (V009539)

LOCUS       V009539                 4558 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009539
VERSION     V009539
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4558)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4558)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..124
                     /label="PMph"
                     /note="PMph"
                     /regulatory_class="promoter"
     misc_feature    74..108
                     /label="MphO operator"
                     /note="MphO operator"
     regulatory      76..81
                     /regulatory_class="minus_35_signal"
     regulatory      99..104
                     /regulatory_class="minus_10_signal"
     regulatory      111
                     /label="+1"
                     /note="+1"
                     /regulatory_class="other"
     misc_RNA        126..176
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      208..219
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             226..942
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      946..1006
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1011..1064
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1029..1034
                     /regulatory_class="minus_35_signal"
     regulatory      1052..1057
                     /regulatory_class="minus_10_signal"
     regulatory      1065..1092
                     /label="mph2"
                     /note="mph2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1093..1677
                     /codon_start=1
                     /product="MphR-AM"
                     /label="MphR-AM"
                     /protein_id="AYJ72267.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMDTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     regulatory      1678..1698
                     /label="ery1"
                     /note="ery1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1699..2430
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     regulatory      2443..2697
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(3009..3554)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3646..4458)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"