LOCUS       V009539                 4558 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009539
VERSION     V009539
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4558)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4558)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..124
                     /label="PMph"
                     /note="PMph"
                     /regulatory_class="promoter"
     misc_feature    74..108
                     /label="MphO operator"
                     /note="MphO operator"
     regulatory      76..81
                     /regulatory_class="minus_35_signal"
     regulatory      99..104
                     /regulatory_class="minus_10_signal"
     regulatory      111
                     /label="+1"
                     /note="+1"
                     /regulatory_class="other"
     misc_RNA        126..176
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      208..219
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             226..942
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      946..1006
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1011..1064
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1029..1034
                     /regulatory_class="minus_35_signal"
     regulatory      1052..1057
                     /regulatory_class="minus_10_signal"
     regulatory      1065..1092
                     /label="mph2"
                     /note="mph2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1093..1677
                     /codon_start=1
                     /product="MphR-AM"
                     /label="MphR-AM"
                     /protein_id="AYJ72267.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMDTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     regulatory      1678..1698
                     /label="ery1"
                     /note="ery1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1699..2430
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     regulatory      2443..2697
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(3009..3554)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3646..4458)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgggattgaa tataaccgac gtgactgtta catttaggtg gctaaacccg
      121 tcaaagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
      181 tacaaataat tttgtttaat actagagaaa gaggggaaat actagatggt gagcaagggc
      241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      361 aagttcatct gcaccacagg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      421 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc
      481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      661 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      781 aacaccccaa tcggcgacgg ccccgtgctg ctgcccgaca accactacct tagctaccag
      841 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      901 accgccgccg ggatcactct cggcatggac gagctgtaca agtaactcgg taccaaattc
      961 cagaaaagag gcctcccgaa aggggggcct tttttcgttt tggtccaatg gcggcgcgcc
     1021 atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattt
     1081 agggaggtga atatgcctcg tccgaaactg aaaagtgatg atgaagttct ggaagcagca
     1141 accgttgttc tgaaacgttg tggtccgatt gaatttaccc tgagcggtgt tgcaaaagaa
     1201 gttggtctga gtcgcgcagc actgattcag cgttttacca atcgtgatac cctgctggtt
     1261 cgtatgatgg aacgtggtgt tgaacaggtt cgtcattatc tgaatgcaat tccgattggt
     1321 gcaggtccgc agggtctgtg ggaatttctg caggttctgg ttcgtagcat ggatacccgt
     1381 aatgatttca gcgtgaacta tctgatcagc tggtatgaac tgcaggttcc ggaactgcgt
     1441 accctggcaa ttcagcgtaa tcgtgcagtt gttgaaggta ttcgtaaacg tctgcctccg
     1501 ggtgcaccgg cagcagcaga actgctgctg catagcgtta ttgccggtgc aaccatgcag
     1561 tgggcagttg atccggatgg tgaactggca gatcatgttc tggcacagat tgcagcaatt
     1621 ctgtgtctga tgtttccgga acatgatgat tttcagctgc tgcaggcaca tgcataataa
     1681 tattgaaaaa ggaagagtat gaacgagaaa aatataaaac acagtcaaaa ctttattact
     1741 tcaaaacata atatagataa aataatgaca aatataagat taaatgaaca tgataatatc
     1801 tttgaaatcg gctcaggaaa aggccatttt acccttgaat tagtaaagag gtgtaatttc
     1861 gtaactgcca ttgaaataga ccataaatta tgcaaaacta cagaaaataa acttgttgat
     1921 cacgataatt tccaagtttt aaacaaggat atattgcagt ttaaatttcc taaaaaccaa
     1981 tcctataaaa tatatggtaa tataccttat aacataagta cggatataat acgcaaaatt
     2041 gtttttgata gtatagctaa tgagatttat ttaatcgtgg aatacgggtt tgctaaaaga
     2101 ttattaaata caaaacgctc attggcatta cttttaatgg cagaagttga tatttctata
     2161 ttaagtatgg ttccaagaga atattttcat cctaaaccta aagtgaatag ctcacttatc
     2221 agattaagta gaaaaaaatc aagaatatca cacaaagata aacaaaagta taattatttc
     2281 gttatgaaat gggttaacaa agaatacaag aaaatattta caaaaaatca atttaacaat
     2341 tccttaaaac atgcaggaat tgacgattta aacaatatta gctttgaaca attcttatct
     2401 cttttcaata gctataaatt atttaataag tgataaggat cctaattggt aacgaatcag
     2461 acaattgacg gctcgaggga gtagcatagg gtttgcagaa tccctgcttc gtccatttga
     2521 caggcacatt atgcatcgat gataagctgt caaacatgag cagatcctct acgccggacg
     2581 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     2641 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcaaatatt ttatctgagg
     2701 tgcttcctcg ctcactgact cgctgcacga ggcagacctc agcgctagcg gagtgtatac
     2761 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     2821 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     2881 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     2941 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     3001 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     3061 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc
     3121 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     3181 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     3241 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct
     3301 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt
     3361 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg
     3421 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct
     3481 tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac
     3541 caaaacgatc tcaagaagat catcttatta aggggtctga cgctcagtgg aacgaaaaat
     3601 caatctaaag tatatatgag taaacttggt ctgacagtta ccttagaaaa actcatcgag
     3661 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     3721 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     3781 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     3841 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     3901 caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     3961 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     4021 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     4081 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     4141 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     4201 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     4261 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     4321 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     4381 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc
     4441 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     4501 tgttcatgat gatatatttt tatcttgtgc aatgtacatc agagattttg agacacaa
//
