pAJ50-2 vector (V009556)

LOCUS       40924_4179        5064 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAJ50-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Heritable genetic transformation of Strongyloides stercoralis by 
            microinjection of plasmid DNA constructs into the male germline
  JOURNAL   Int. J. Parasitol. (2017) In press
  PUBMED    28577882
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2017) Pathobiology, University of Pennsylvania, 
            3800 Spruce Street, Rosenthal 212, Philadelphia, PA 19104, USA
REFERENCE   3  (bases 1 to 5064)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int. J. 
            Parasitol. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2017) Pathobiology, University of Pennsylvania, 3800 Spruce 
            Street, Rosenthal 212, Philadelphia, PA 19104, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    96..117
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        132..162
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    170..186
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      346..1508
                     /regulatory_class="promoter"
     CDS             1513..2187
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     3'UTR           2212..2763
                     /gene="Ss era-1"
     gene            2212..2763
                     /gene="Ss era-1"
                     /label=Ss era-1
     promoter        3148..3252
                     /label=AmpR promoter
     CDS             3253..4110
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"