LOCUS       40924_4179        5064 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAJ50-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Heritable genetic transformation of Strongyloides stercoralis by 
            microinjection of plasmid DNA constructs into the male germline
  JOURNAL   Int. J. Parasitol. (2017) In press
  PUBMED    28577882
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2017) Pathobiology, University of Pennsylvania, 
            3800 Spruce Street, Rosenthal 212, Philadelphia, PA 19104, USA
REFERENCE   3  (bases 1 to 5064)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int. J. 
            Parasitol. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2017) Pathobiology, University of Pennsylvania, 3800 Spruce 
            Street, Rosenthal 212, Philadelphia, PA 19104, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    96..117
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        132..162
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    170..186
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      346..1508
                     /regulatory_class="promoter"
     CDS             1513..2187
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     3'UTR           2212..2763
                     /gene="Ss era-1"
     gene            2212..2763
                     /gene="Ss era-1"
                     /label=Ss era-1
     promoter        3148..3252
                     /label=AmpR promoter
     CDS             3253..4110
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
       61 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      121 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      181 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctgta agtttaaaca
      241 tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
      301 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttaccat gatattaaat
      361 aagtgaaagt tttagtggac ttttgatgaa tgtgatgttt ttgttaaaga ttttctttta
      421 aatactaatt tttcaatttt taaaaacttg ctttttaggt aataagattg tttaaggtta
      481 aacgaggcat taaatcaaat actagacatt ttataacatc taccgattta tcgtcttaag
      541 tcatatcctc atttatctca ttaacatatc cagatactaa tgttatatat aaattatcat
      601 tgttattata tatatattta ttgtactttt attgtagttt tcacttttta ataaaaatat
      661 aaattgattc cagaattgat tttctaatta caatttactt ttgatacatc aaatactatt
      721 ctttatgaca tctagtttta aaggatattt atgtaataca attattgctt gattgcttta
      781 ttgtaattaa attatagtcc atttaaaaaa aaaaaatttt ttttatatat atattcaatt
      841 aattaaaaaa atgttccaaa tgaatttata ttattacaag atataaaatg gtatagaaat
      901 tttgttatta aaaattttaa atttcatttt ataaacaaaa agtctttgaa aacaaataaa
      961 actaacacaa tacttttatt taatcaaata caatagatct ttatatttgt gcttttaagt
     1021 tattaacaaa tatttcaatt ttcataaaat acttcatttt tgaatttatg taaatttttt
     1081 taaaaggcat acttttagcg attgatagtt aacatattca cgtcagtgcg acctcaaatt
     1141 ttttccacta gtacttatct ataatgataa aaaaaaaaga ggggtgttta ccgaaaagtt
     1201 ttaatagcca atatacgtga tgtgcaggct tttttacgtt tttattaccg taaaaagtat
     1261 agcgttttgg agatttattt ataattagta tacataatgc cgatgctcag ttatgtttct
     1321 taaccagaat aacctgaatg tatagacatt tttccacaac ccaaatgtta tattaacatt
     1381 tatatttaat tgttatatat tttcatccca cgttgcattt tagatttaat ttttatatat
     1441 ttgaaaatat attttaatat aattattttt atttaattat agattcatta gctatttacc
     1501 ataccggtaa aaatggcatc atcagaagat gttattaaag aatttatgag attcaaagtt
     1561 aaaatggaag gttcagttaa cggtcatgaa tttgaaattg aaggtgaagg tgaaggtaga
     1621 ccatatgaag gtacacaaac agcaaaatta aaagttacaa aaggtggtcc attaccattt
     1681 gcatgggata ttctttcacc acaatttcaa tatggtagta aagcatatgt taaacatcca
     1741 gcagatattc cagattatct taaactttca tttccagaag gttttaaatg ggaaagaatt
     1801 atgaattttg aagatggtgg tgttgtaaca gttactcaag attcaacatt acaagatggt
     1861 gaatttattt ataaagttaa attaagaggt accaattttc catcagatgg tccagtaatg
     1921 caaaaaaaaa ctatgggttg ggaagcatca acagaaagaa tgtatccaga agatggtgca
     1981 ctaaaaggtg aaattaaaat gagattaaaa cttaaggatg gtggtcatta tgatgcagaa
     2041 gttaaaacaa cttataaagc taaaaaacaa gttcaattac caggtgctta taaaacagat
     2101 attaaattag atattatttc acataatgaa gattatacta ttgttgaaca atatgaaaga
     2161 gcagaaggta gacattcaac aggtgcagga tctcctagga atttattttg ataagtctcg
     2221 cgtattttat ttgtaataat nttataataa gatcaattta catttatact gataaaattg
     2281 acacaattac actttttatt ataaaatatt ttaaaatatt tctatcaaaa ggtttatttt
     2341 tgttttaaat ataatggttt tttttgtaaa aaatacttat ttcgtttgat aatctttatt
     2401 cagagatttg aataatttta aataaagtag tttagttttc atataaaagt taatggtata
     2461 tataaatatt taaatagtaa agattgaatc agtattcaaa atgacaccta aaagttattc
     2521 tatttctagc tataacaata ataataaaga taattatgat acagatttag aaaaaaaaat
     2581 aaggttaaat taattaattc tatttttaat taaaatgatt taagggagtt aaatgaaaca
     2641 gaaacagatt gggtctctat atatttagtt tatgtaatgt tattttttgg tggaatacaa
     2701 ataacggtat attttacttc aatgtggcca tacttatcaa caataaatag taaggcaaat
     2761 gtagatactt tagggtgggt tgtagcacta gtcggccgta cgggcccttt cgtctcgcgc
     2821 gtttcggtga tgacggtgaa aacctctgac acatgcagct agcggagacg gtcacagctt
     2881 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2941 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     3001 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag
     3061 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3121 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3241 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3301 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3361 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3421 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3481 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3541 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3601 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3661 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3721 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3781 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3841 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3901 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3961 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4021 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4081 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4981 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5041 gaggaagcgg aagagcgccc aata
//
