pAGA3 vector (V009571)

LOCUS       40924_4105        3018 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAGA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Hu HZ, Gu Q, Wang C, Colton CK, Tang J, Kinoshita-Kawada M, Lee LY, 
            Wood JD, Zhu MX.
  TITLE     2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, 
            and TRPV3
  JOURNAL   J. Biol. Chem. 279 (34), 35741-35748 (2004)
  PUBMED    15194687
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Zhu MX.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2003) Neuroscience, The Ohio State University, 168
            Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 3018)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2004"; volume: "279"; issue: "34"; pages: 
            "35741-35748"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2003) Neuroscience, The Ohio State University, 168 Rightmire
            Hall, 1060 Carmack Road, Columbus, OH 43210, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    33..66
                     /note="5' untranslated region of alfalfa mosaic virus RNA
                     4"
     misc_feature    67..114
                     /label=polylinker
                     /note="polylinker"
     misc_feature    115..206
                     /label=polyA tail
                     /note="polyA tail"
     promoter        complement(239..257)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1043..1147
                     /label=AmpR promoter
     CDS             1148..2005
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2179..2767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"