LOCUS       40924_4105        3018 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAGA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Hu HZ, Gu Q, Wang C, Colton CK, Tang J, Kinoshita-Kawada M, Lee LY, 
            Wood JD, Zhu MX.
  TITLE     2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, 
            and TRPV3
  JOURNAL   J. Biol. Chem. 279 (34), 35741-35748 (2004)
  PUBMED    15194687
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Zhu MX.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2003) Neuroscience, The Ohio State University, 168
            Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 3018)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2004"; volume: "279"; issue: "34"; pages: 
            "35741-35748"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2003) Neuroscience, The Ohio State University, 168 Rightmire
            Hall, 1060 Carmack Road, Columbus, OH 43210, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    33..66
                     /note="5' untranslated region of alfalfa mosaic virus RNA
                     4"
     misc_feature    67..114
                     /label=polylinker
                     /note="polylinker"
     misc_feature    115..206
                     /label=polyA tail
                     /note="polyA tail"
     promoter        complement(239..257)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1043..1147
                     /label=AmpR promoter
     CDS             1148..2005
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2179..2767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taatacgact cactataggg agaccggaat tcgtttttat ttttaatttt ctttcaaata
       61 cttccaccat ggatatcgat ggatcccggg tcgacggtac ctctagataa ctagaaaaaa
      121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      181 aaaaaaaaaa aaaaaaaaaa aaaaaagctt ctcgagctgc aggcatgcaa gcttgtattc
      241 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
      301 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
      361 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
      421 ctggtaacgc cgttccgcac cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
      481 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      541 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      601 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      661 gccatctcct tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
      721 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg atatggtgca ctctcagtac
      781 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
      841 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      901 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
      961 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1021 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1081 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1141 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1201 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1261 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1321 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1381 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1441 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1501 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1561 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1621 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1681 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1741 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1801 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1861 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1921 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1981 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2041 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2101 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2161 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2221 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2281 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2341 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2401 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2461 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2521 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2581 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2641 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2701 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2761 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2821 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2881 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2941 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3001 cagctggctt atcgaaat
//
