Basic Vector Information
- Vector Name:
- pADIS1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5106 bp
- Type:
- Cloning vector
- Replication origin:
- oriV
- Source/Author:
- Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
pADIS1 vector Map
pADIS1 vector Sequence
LOCUS V009576 5106 bp DNA circular SYN 17-DEC-2018
DEFINITION Exported.
ACCESSION V009576
VERSION V009576
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5106)
AUTHORS Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
TITLE GABI-Kat SimpleSearch: a flanking sequence tag (FST) database for
the identification of T-DNA insertion mutants in Arabidopsis
thaliana
JOURNAL Bioinformatics 19 (11), 1441-1442 (2003)
PUBMED 12874060
REFERENCE 2 (bases 1 to 5106)
AUTHORS Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B.
TITLE An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for
flanking sequence tag-based reverse genetics
JOURNAL Plant Mol. Biol. 53 (1-2), 247-259 (2003)
PUBMED 14756321
REFERENCE 3 (bases 1 to 5106)
AUTHORS Rosso MG, Li Y, Strizhov N, Weisshaar B.
TITLE Direct Submission
JOURNAL Submitted (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for
Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829,
Germany
REFERENCE 4 (bases 1 to 5106)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 5106)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Bioinformatics"; date: "2003"; volume: "19"; issue: "11"; pages:
"1441-1442"
SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol.
Biol. 53 (1-2), 247-259 (2003)"
SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(20-JAN-2004) Dept. Salamini, Max-Planck-Institute for Plant
Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, Germany"
SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5106
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 31..740
/label="oriV"
/note="incP origin of replication"
promoter 813..904
/label="AmpR promoter"
CDS 905..1762
/label="AmpR"
/note="beta-lactamase"
rep_origin 1936..2524
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2832..2856
/label="LB T-DNA repeat"
/note="left border repeat from octopine Ach5 T-DNA"
regulatory 3129..3517
/label="eucaryotic promoter ubi 4-2#"
/note="eucaryotic promoter ubi 4-2#"
/regulatory_class="promoter"
regulatory 3512..3521
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 3518..3688
/label="pea rubisco targeting signal"
/note="pea rubisco targeting signal"
/regulatory_class="other"
CDS 3689..4525
/gene="sulI"
/label="Dihydropteroate synthase type-1"
/note="Dihydropteroate synthase type-1 from Escherichia
coli. Accession#: P0C002"
terminator 4573..4825
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 5083..5106
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
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