LOCUS       V009576                 5106 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009576
VERSION     V009576
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
  TITLE     GABI-Kat SimpleSearch: a flanking sequence tag (FST) database for
            the identification of T-DNA insertion mutants in Arabidopsis
            thaliana
  JOURNAL   Bioinformatics 19 (11), 1441-1442 (2003)
   PUBMED   12874060
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B.
  TITLE     An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for
            flanking sequence tag-based reverse genetics
  JOURNAL   Plant Mol. Biol. 53 (1-2), 247-259 (2003)
   PUBMED   14756321
REFERENCE   3  (bases 1 to 5106)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Weisshaar B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829,
            Germany
REFERENCE   4  (bases 1 to 5106)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Bioinformatics"; date: "2003"; volume: "19"; issue: "11"; pages:
            "1441-1442"
            SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol.
            Biol. 53 (1-2), 247-259 (2003)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for Plant
            Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, Germany"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..740
                     /label="oriV"
                     /note="incP origin of replication"
     promoter        813..904
                     /label="AmpR promoter"
     CDS             905..1762
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2832..2856
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      3129..3517
                     /label="eucaryotic promoter ubi 4-2#"
                     /note="eucaryotic promoter ubi 4-2#"
                     /regulatory_class="promoter"
     regulatory      3512..3521
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3518..3688
                     /label="pea rubisco targeting signal"
                     /note="pea rubisco targeting signal"
                     /regulatory_class="other"
     CDS             3689..4525
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     terminator      4573..4825
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5083..5106
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ctcgagcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg
       61 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
      121 agccgtgtgc gagacaccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
      181 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
      241 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
      301 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
      361 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
      421 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
      481 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
      541 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
      601 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
      661 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
      721 cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg gggcagtaac
      781 gggatgggcg atcagcccga aagctacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      961 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1021 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1081 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1141 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1201 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1261 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1321 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1381 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1441 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1501 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1561 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1621 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1681 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1741 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1801 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1861 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2041 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2581 catggactct agaggatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     2641 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     2701 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     2761 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     2821 tggctctcga gcggcaggat atattcaatt gtaaatggct tcatgtccgg gaaatctaca
     2881 tggatcagca atgagtatga tggtcaatat ggagaaaaag aaagagtaat taccaatttt
     2941 ttttcaattc aaaaatgtag atgtccgcag cgttattata aaatgaaagt acattttgat
     3001 aaaacgacaa attacgatcc gtcgtattta taggcgaaag caataaacaa attattctaa
     3061 ttcggaaatc tttatttcga cgtgtctaca ttcacgtcca aatgggggct tagatgagaa
     3121 acttcacgat cgatgaattc gaatccaaaa attacggata tgaatatagg catatccgta
     3181 tccgaattat ccgtttgaca gctagcaacg attgtacaat tgcttcttta aaaaaggaag
     3241 aaagaaagaa agaaaagaat caacatcagc gttaacaaac ggccccgtta cggcccaaac
     3301 ggtcatatag agtaacggcg ttaagcgttg aaagactcct atcgaaatac gtaaccgcaa
     3361 acgtgtcata gtcagatccc ctcttccttc accgcctcaa acacaaaaat aatcttctac
     3421 agcctatata tacaaccccc ccttctatct ctcctttctc acaattcatc atctttcttt
     3481 ctctaccccc aattttaaga aatctcgagt ggccaccatg gcttctatga tatcctcttc
     3541 agctgtgact acagtcagcc gtgcttctac ggtgcaatcg gccgcggtgg ctccattcgg
     3601 cggcctcaaa tccatgactg gattcccagt taagaaggtc aacactgaca ttacttccat
     3661 tacaagcaat ggtggaagag taaagtgcat ggtgacggtg ttcggcattc tgaatctcac
     3721 cgaggactcc ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc
     3781 gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc
     3841 ggacgcgagg cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc
     3901 cctgtccgat cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta
     3961 tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct
     4021 ctatcccgat attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga
     4081 tggcatcgcc acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg
     4141 gttcttcgag gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat
     4201 cctcgatccg gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct
     4261 gtcgaacctt caaaagctga agtcggcgtt ggggcttccg ctattggtct cggtgtcgcg
     4321 gaaatccttc ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct
     4381 tgcggcggaa cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg
     4441 agatctgcga agcgcaatca ccatctcgga aaccctcgcg aaatttcgca gtcgcgacgc
     4501 cagagaccga gggttagatc atgcctagag ctcccgggta cctgatcatg agtaattagc
     4561 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     4621 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     4681 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     4741 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     4801 gcggtgtcat ctatgttact agatcgggaa ttagatctgc tagcaggtgg cacttttcgg
     4861 ggaaatgtcg acctgtacag gcatgcccgc tgaaatcacc agtctctctc tacaaatcta
     4921 tctctctcta taataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg
     4981 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     5041 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca ggtgacagga tatattggcg
     5101 ggtaaa
//
