pCC-ccdB vector (V008677)

LOCUS       V008677                 9670 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008677
VERSION     V008677
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Lu T.
  TITLE     Spatial Interference Scale as a Determinant of Microbial Community
            Assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 9670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(340..1089)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     regulatory      complement(1198..1253)
                     /label="promoter PluxR"
                     /note="promoter PluxR"
                     /regulatory_class="promoter"
     regulatory      1234..1292
                     /label="promoter PluxI"
                     /note="promoter PluxI"
                     /regulatory_class="promoter"
     CDS             1336..1629
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      1647..1759
                     /label="T1 terminator"
                     /note="T1 terminator"
                     /regulatory_class="terminator"
     promoter        1853..1871
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1874..1930
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1973..1989)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1997..2013)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2021..2051)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2066..2087)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2310..2966)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2967..3069)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3928..4542
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4618..4837
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             4928..5680
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    5686..5936
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6262..7434
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             7437..8405
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    8481..8954
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(9214..9612)
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     protein_bind    complement(9630..9663)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."