LOCUS       V008677                 9670 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008677
VERSION     V008677
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Lu T.
  TITLE     Spatial Interference Scale as a Determinant of Microbial Community
            Assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 9670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(340..1089)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     regulatory      complement(1198..1253)
                     /label="promoter PluxR"
                     /note="promoter PluxR"
                     /regulatory_class="promoter"
     regulatory      1234..1292
                     /label="promoter PluxI"
                     /note="promoter PluxI"
                     /regulatory_class="promoter"
     CDS             1336..1629
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      1647..1759
                     /label="T1 terminator"
                     /note="T1 terminator"
                     /regulatory_class="terminator"
     promoter        1853..1871
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1874..1930
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1973..1989)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1997..2013)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2021..2051)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2066..2087)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2310..2966)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2967..3069)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3928..4542
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4618..4837
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             4928..5680
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    5686..5936
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6262..7434
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             7437..8405
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    8481..8954
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(9214..9612)
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     protein_bind    complement(9630..9663)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccacacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 acatttcccc gaaaagtgcc acctgacgtc ctcgagttaa tttttaaagt atgggcaatc
      361 aattgctcct gttaaaattg ctttagaaat actttggcag cggtttgttg tattgagttt
      421 catttgcgca ttggttaaat ggaaagtgac agtacgctca ctgcagccta atatttttga
      481 aatatcccaa gagctttttc cttcgcatgc ccacgctaaa cattcttttt ctcttttggt
      541 taaatcgttg tttgatttat tatttgctat atttattttt cgataattat caactagaga
      601 aggaacaatt aatggtatgt tcatacacgc atgtaaaaat aaactatcta tatagttgtc
      661 tttttctgaa tgtgcaaaac taagcattcc gaagccattg ttagccgtat gaatagggaa
      721 actaaaccca gtgataagac ctgatgtttt cgcttcttta attacatttg gagatttttt
      781 atttacagca ttgttttcaa atatattcca attaattggt gaatgattgg agttagaata
      841 atctactata ggatcatatt ttattaaatt agcgtcatca taatattgcc tccatttttt
      901 agggtaatta tctagaattg aaatatcaga tttaaccata gaatgaggat aaatgatcgc
      961 gagtaaataa tattcacaat gtaccatttt agtcatatca gataagcatt gattaatatc
     1021 attattgctt ctacaagctt taattttatt aattattctg tatgtgtcgt cggcatttat
     1081 gtttttcata cccatctctt tatccttacc tattgtttgt cgcaagtttt gcgtgttata
     1141 tatcattaaa acggtaatgg attgacattt gattctaata aattggattt ttgtcacact
     1201 attgtatcgc tgggaataca attacttaac ataagcacct gtaggatcgt acaggtttac
     1261 gcaagaaaat ggtttgttat agtcgaatga atctaaaaat ataccattac cgaaggaaga
     1321 gtaaattatg cagttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     1381 gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt
     1441 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     1501 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     1561 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     1621 tggggaatat aattggggtt gaataaccgg cttatcggtc agtttcacct gatttacgta
     1681 aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg actgtccacg
     1741 acgctatacc caaaagaaaa tctattgtac taatcggctt caacgtgccg cacgggtggc
     1801 acctcaggag ggatatattc cgcttcctcg ctcactgact cgcagtgaat tgtaatacga
     1861 ctcactatag ggcgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc
     1921 atgcaagctt gagtattcta tagtctcacc taaatagctt ggcgtaatca tggtcatagc
     1981 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2041 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2101 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2161 gcgaacccct tgcggccgcc cgggccgtcg accaattctc atgtttgaca gcttatcatc
     2221 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa
     2281 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     2341 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     2401 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     2461 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     2521 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     2581 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     2641 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     2701 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     2761 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     2821 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     2881 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     2941 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     3001 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     3061 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     3121 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcgcgataa
     3181 gctcatggag cggcgtaacc gtcgcacagg aaggacagag aaagcgcgga tctgggaagt
     3241 gacggacaga acggtcagga cctggattgg ggaggcggtt gccgccgctg ctgctgacgg
     3301 tgtgacgttc tctgttccgg tcacaccaca tacgttccgc cattcctatg cgatgcacat
     3361 gctgtatgcc ggtataccgc tgaaagttct gcaaagcctg atgggacata agtccatcag
     3421 ttcaacggaa gtctacacga aggtttttgc gctggatgtg gctgcccggc accgggtgca
     3481 gtttgcgatg ccggagtctg atgcggttgc gatgctgaaa caattatcct gagaataaat
     3541 gccttggcct ttatatggaa atgtggaact gagtggatat gctgtttttg tctgttaaac
     3601 agagaagctg gctgttatcc actgagaagc gaacgaaaca gtcgggaaaa tctcccatta
     3661 tcgtagagat ccgcattatt aatctcagga gcctgtgtag cgtttatagg aagtagtgtt
     3721 ctgtcatgat gcctgcaagc ggtaacgaaa acgatttgaa tatgccttca ggaacaatag
     3781 aaatcttcgt gcggtgttac gttgaagtgg agcggattat gtcagcaatg gacagaacaa
     3841 cctaatgaac acagaaccat gatgtggtct gtccttttac agccagtagt gctcgccgca
     3901 gtcgagcgac agggcgaagc cctcgagctg gttgccctcg ccgctgggct ggcggccgtc
     3961 tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgg
     4021 ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga
     4081 cgttgacact tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg
     4141 atttcggccg gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta
     4201 cgcgagtttc ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca
     4261 cttgaggggc gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag
     4321 tgctgacaga tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc
     4381 cagcatttgc aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc
     4441 ttttaaacca atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc
     4501 gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggtcgagt gagcgaggaa
     4561 gcaccaggga acagcactta tatattctgc ttacacacga tgcctgaaaa aacttccctt
     4621 ggggttatcc acttatccac ggggatattt ttataattat tttttttata gtttttagat
     4681 cttctttttt agagcgcctt gtaggccttt atccatgctg gttctagaga aggtgttgtg
     4741 acaaattgcc ctttcagtgt gacaaatcac cctcaaatga cagtcctgtc tgtgacaaat
     4801 tgcccttaac cctgtgacaa attgccctca gaagaagctg ttttttcaca aagttatccc
     4861 tgcttattga ctctttttta tttagtgtga caatctaaaa acttgtcaca cttcacatgg
     4921 atctgtcatg gcggaaacag cggttatcaa tcacaagaaa cgtaaaaata gcccgcgaat
     4981 cgtccagtca aacgacctca ctgaggcggc atatagtctc tcccgggatc aaaaacgtat
     5041 gctgtatctg ttcgttgacc agatcagaaa atctgatggc accctacagg aacatgacgg
     5101 tatctgcgag atccatgttg ctaaatatgc tgaaatattc ggattgacct ctgcggaagc
     5161 cagtaaggat atacggcagg cattgaagag tttcgcgggg aaggaagtgg ttttttatcg
     5221 ccctgaagag gatgccggcg atgaaaaagg ctatgaatct tttccttggt ttatcaaacg
     5281 tgcgcacagt ccatccagag ggctttacag tgtacatatc aacccatatc tcattccctt
     5341 ctttatcggg ttacagaacc ggtttacgca gtttcggctt agtgaaacaa aagaaatcac
     5401 caatccgtat gccatgcgtt tatacgaatc cctgtgtcag tatcgtaagc cggatggctc
     5461 aggcatcgtc tctctgaaaa tcgactggat catagagcgt taccagctgc ctcaaagtta
     5521 ccagcgtatg cctgacttcc gccgccgctt cctgcaggtc tgtgttaatg agatcaacag
     5581 cagaactcca atgcgcctct catacattga gaaaaagaaa ggccgccaga cgactcatat
     5641 cgtattttcc ttccgcgata tcacttccat gacgacagga tagtctgagg gttatctgtc
     5701 acagatttga gggtggttcg tcacatttgt tctgacctac tgagggtaat ttgtcacagt
     5761 tttgctgttt ccttcagcct gcatggattt tctcatactt tttgaactgt aatttttaag
     5821 gaagccaaat ttgagggcag tttgtcacag ttgatttcct tctctttccc ttcgtcatgt
     5881 gacctgatat cgggggttag ttcgtcatca ttgatgaggg ttgattatca cagtttatta
     5941 ctctgaattg gctatccgcg tgtgtacctc tacctggagt ttttcccacg gtggatattt
     6001 cttcttgcgc tgagcgtaag agctatctga cagaacagtt cttctttgct tcctcgccag
     6061 ttcgctcgct atgctcggtt acacggctgc ggcgagcgct agtgataata agtgactgag
     6121 gtatgtgctc ttcttatctc cttttgtagt gttgctctta ttttaaacaa ctttgcggtt
     6181 ttttgatgac tttgcgattt tgttgttgct ttgcagtaaa ttgcaagatt taataaaaaa
     6241 acgcaaagca atgattaaag gatgttcaga atgaaactca tggaaacact taaccagtgc
     6301 ataaacgctg gtcatgaaat gacgaaggct atcgccattg cacagtttaa tgatgacagc
     6361 ccggaagcga ggaaaataac ccggcgctgg agaataggtg aagcagcgga tttagttggg
     6421 gtttcttctc aggctatcag agatgccgag aaagcagggc gactaccgca cccggatatg
     6481 gaaattcgag gacgggttga gcaacgtgtt ggttatacaa ttgaacaaat taatcatatg
     6541 cgtgatgtgt ttggtacgcg attgcgacgt gctgaagacg tatttccacc ggtgatcggg
     6601 gttgctgccc ataaaggtgg cgtttacaaa acctcagttt ctgttcatct tgctcaggat
     6661 ctggctctga aggggctacg tgttttgctc gtggaaggta acgaccccca gggaacagcc
     6721 tcaatgtatc acggatgggt accagatctt catattcatg cagaagacac tctcctgcct
     6781 ttctatcttg gggaaaagga cgatgtcact tatgcaataa agcccacttg ctggccgggg
     6841 cttgacatta ttccttcctg tctggctctg caccgtattg aaactgagtt aatgggcaaa
     6901 tttgatgaag gtaaactgcc caccgatcca cacctgatgc tccgactggc cattgaaact
     6961 gttgctcatg actatgatgt catagttatt gacagcgcgc ctaacctggg tatcggcacg
     7021 attaatgtcg tatgtgctgc tgatgtgctg attgttccca cgcctgctga gttgtttgac
     7081 tacacctccg cactgcagtt tttcgatatg cttcgtgatc tgctcaagaa cgttgatctt
     7141 aaagggttcg agcctgatgt acgtattttg cttaccaaat acagcaatag caatggctct
     7201 cagtccccgt ggatggagga gcaaattcgg gatgcctggg gaagcatggt tctaaaaaat
     7261 gttgtacgtg aaacggatga agttggtaaa ggtcagatcc ggatgagaac tgtttttgaa
     7321 caggccattg atcaacgctc ttcaactggt gcctggagaa atgctctttc tatttgggaa
     7381 cctgtctgca atgaaatttt cgatcgtctg attaaaccac gctgggagat tagataatga
     7441 agcgtgcgcc tgttattcca aaacatacgc tcaatactca accggttgaa gatacttcgt
     7501 tatcgacacc agctgccccg atggtggatt cgttaattgc gcgcgtagga gtaatggctc
     7561 gcggtaatgc cattactttg cctgtatgtg gtcgggatgt gaagtttact cttgaagtgc
     7621 tccggggtga tagtgttgag aagacctctc gggtatggtc aggtaatgaa cgtgaccagg
     7681 agctgcttac tgaggacgca ctggatgatc tcatcccttc ttttctactg actggtcaac
     7741 agacaccggc gttcggtcga agagtatctg gtgtcataga aattgccgat gggagtcgcc
     7801 gtcgtaaagc tgctgcactt accgaaagtg attatcgtgt tctggttggc gagctggatg
     7861 atgagcagat ggctgcatta tccagattgg gtaacgatta tcgcccaaca agtgcttatg
     7921 aacgtggtca gcgttatgca agccgattgc agaatgaatt tgctggaaat atttctgcgc
     7981 tggctgatgc ggaaaatatt tcacgtaaga ttattacccg ctgtatcaac accgccaaat
     8041 tgcctaaatc agttgttgct cttttttctc accccggtga actatctgcc cggtcaggtg
     8101 atgcacttca aaaagccttt acagataaag aggaattact taagcagcag gcatctaacc
     8161 ttcatgagca gaaaaaagct ggggtgatat ttgaagctga agaagttatc actcttttaa
     8221 cttctgtgct taaaacgtca tctgcatcaa gaactagttt aagctcacga catcagtttg
     8281 ctcctggagc gacagtattg tataagggcg ataaaatggt gcttaacctg gacaggtctc
     8341 gtgttccaac tgagtgtata gagaaaattg aggccattct taaggaactt gaaaagccag
     8401 caccctgatg cgaccacgtt ttagtctacg tttatctgtc tttacttaat gtcctttgtt
     8461 acaggccaga aagcataact ggcctgaata ttctctctgg gcccactgtt ccacttgtat
     8521 cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta
     8581 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc
     8641 cactcgtatc gtcggtctga taatcagact gggaccacgg tcccactcgt atcgtcggtc
     8701 tgattattag tctgggacca tggtcccact cgtatcgtcg gtctgattat tagtctggga
     8761 ccacggtccc actcgtatcg tcggtctgat tattagtctg gaaccacggt cccactcgta
     8821 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     8881 agtctgggac cacgatccca ctcgtgttgt cggtctgatt atcggtctgg gaccacggtc
     8941 ccacttgtat tgtcgatcag actatcagcg tgagactacg attccatcaa tgcctgtcaa
     9001 gggcaagtat tgacatgtcg tcgtaacctg tagaacggag taacctcggt gtgcggttgt
     9061 atgcctgctg tggattgctg ctgtgtcctg cttatccaca acattttgcg cacggttatg
     9121 tggacaaaat acctggttac ccaggccgtg ccggcacgtt aaccgggctg catccgatgc
     9181 aagtgtgtcg ctgtcgacga gctcgcgagc tcggacatga ggttgccccg tattcagtgt
     9241 cgctgatttg tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg
     9301 atacctgcgt cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata
     9361 tgtagatgat aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg
     9421 cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc
     9481 ttcttcgtca taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca
     9541 ggtgctgaaa gcgagctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa
     9601 tgaacaatgg aagtccgagc tcatcgctaa taacttcgta tagcatacat tatacgaagt
     9661 tatattcgat
//
