pCC-AHL vector (V008678)

LOCUS       V008678                 9052 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008678
VERSION     V008678
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Lu T.
  TITLE     Spatial Interference Scale as a Determinant of Microbial Community
            Assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 9052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        331..360
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     RBS             386..394
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             400..978
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             979..1011
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     regulatory      1029..1141
                     /label="T1 terminator"
                     /note="T1 terminator"
                     /regulatory_class="terminator"
     promoter        1235..1253
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1256..1312
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1355..1371)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1379..1395)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1403..1433)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1448..1469)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1692..2348)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2349..2451)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3310..3924
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4000..4219
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             4310..5062
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    5068..5318
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             5644..6816
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             6819..7787
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    7863..8336
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(8596..8994)
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     protein_bind    complement(9012..9045)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."