LOCUS       V008678                 9052 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008678
VERSION     V008678
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Lu T.
  TITLE     Spatial Interference Scale as a Determinant of Microbial Community
            Assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 9052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-JAN-2018) Bioengineering, University of Illinois at
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        331..360
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     RBS             386..394
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             400..978
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             979..1011
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     regulatory      1029..1141
                     /label="T1 terminator"
                     /note="T1 terminator"
                     /regulatory_class="terminator"
     promoter        1235..1253
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1256..1312
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1355..1371)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1379..1395)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1403..1433)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1448..1469)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1692..2348)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2349..2451)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3310..3924
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4000..4219
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             4310..5062
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    5068..5318
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             5644..6816
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             6819..7787
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    7863..8336
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(8596..8994)
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     protein_bind    complement(9012..9045)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccacacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 acatttcccc gaaaagtgcc acctgacgtc ttgacaatta atcatccggc tcgtataatg
      361 tgtggggcgg atcctagcct aacaataagg aggtagacta tgactataat gataaaaaaa
      421 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa
      481 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag
      541 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga
      601 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg
      661 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta
      721 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa
      781 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca
      841 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa
      901 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt
      961 aaaaaagcag tcttaaatgc agcgaacgac gaaaattacg cccttgcagc gtaattgggg
     1021 ttgaataacc ggcttatcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg
     1081 tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata cccaaaagaa
     1141 aatctattgt actaatcggc ttcaacgtgc cgcacgggtg gcacctcagg agggatatat
     1201 tccgcttcct cgctcactga ctcgcagtga attgtaatac gactcactat agggcgaatt
     1261 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgagtattc
     1321 tatagtctca cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1381 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1441 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1501 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgaaccc cttgcggccg
     1561 cccgggccgt cgaccaattc tcatgtttga cagcttatca tcgaatttct gccattcatc
     1621 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     1681 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     1741 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1801 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1861 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     1921 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1981 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2041 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2101 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     2161 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2221 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2281 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2341 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2401 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     2461 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     2521 tctgcgaagt gatcttccgt cacaggtatt tattcgcgat aagctcatgg agcggcgtaa
     2581 ccgtcgcaca ggaaggacag agaaagcgcg gatctgggaa gtgacggaca gaacggtcag
     2641 gacctggatt ggggaggcgg ttgccgccgc tgctgctgac ggtgtgacgt tctctgttcc
     2701 ggtcacacca catacgttcc gccattccta tgcgatgcac atgctgtatg ccggtatacc
     2761 gctgaaagtt ctgcaaagcc tgatgggaca taagtccatc agttcaacgg aagtctacac
     2821 gaaggttttt gcgctggatg tggctgcccg gcaccgggtg cagtttgcga tgccggagtc
     2881 tgatgcggtt gcgatgctga aacaattatc ctgagaataa atgccttggc ctttatatgg
     2941 aaatgtggaa ctgagtggat atgctgtttt tgtctgttaa acagagaagc tggctgttat
     3001 ccactgagaa gcgaacgaaa cagtcgggaa aatctcccat tatcgtagag atccgcatta
     3061 ttaatctcag gagcctgtgt agcgtttata ggaagtagtg ttctgtcatg atgcctgcaa
     3121 gcggtaacga aaacgatttg aatatgcctt caggaacaat agaaatcttc gtgcggtgtt
     3181 acgttgaagt ggagcggatt atgtcagcaa tggacagaac aacctaatga acacagaacc
     3241 atgatgtggt ctgtcctttt acagccagta gtgctcgccg cagtcgagcg acagggcgaa
     3301 gccctcgagc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     3361 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc ggccgccggc gttgtggata
     3421 cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc
     3481 cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg
     3541 gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat
     3601 gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac
     3661 tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc
     3721 gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt
     3781 ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat
     3841 aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg
     3901 tgccccccct tctcgaaccc tcccggtcga gtgagcgagg aagcaccagg gaacagcact
     3961 tatatattct gcttacacac gatgcctgaa aaaacttccc ttggggttat ccacttatcc
     4021 acggggatat ttttataatt atttttttta tagtttttag atcttctttt ttagagcgcc
     4081 ttgtaggcct ttatccatgc tggttctaga gaaggtgttg tgacaaattg ccctttcagt
     4141 gtgacaaatc accctcaaat gacagtcctg tctgtgacaa attgccctta accctgtgac
     4201 aaattgccct cagaagaagc tgttttttca caaagttatc cctgcttatt gactcttttt
     4261 tatttagtgt gacaatctaa aaacttgtca cacttcacat ggatctgtca tggcggaaac
     4321 agcggttatc aatcacaaga aacgtaaaaa tagcccgcga atcgtccagt caaacgacct
     4381 cactgaggcg gcatatagtc tctcccggga tcaaaaacgt atgctgtatc tgttcgttga
     4441 ccagatcaga aaatctgatg gcaccctaca ggaacatgac ggtatctgcg agatccatgt
     4501 tgctaaatat gctgaaatat tcggattgac ctctgcggaa gccagtaagg atatacggca
     4561 ggcattgaag agtttcgcgg ggaaggaagt ggttttttat cgccctgaag aggatgccgg
     4621 cgatgaaaaa ggctatgaat cttttccttg gtttatcaaa cgtgcgcaca gtccatccag
     4681 agggctttac agtgtacata tcaacccata tctcattccc ttctttatcg ggttacagaa
     4741 ccggtttacg cagtttcggc ttagtgaaac aaaagaaatc accaatccgt atgccatgcg
     4801 tttatacgaa tccctgtgtc agtatcgtaa gccggatggc tcaggcatcg tctctctgaa
     4861 aatcgactgg atcatagagc gttaccagct gcctcaaagt taccagcgta tgcctgactt
     4921 ccgccgccgc ttcctgcagg tctgtgttaa tgagatcaac agcagaactc caatgcgcct
     4981 ctcatacatt gagaaaaaga aaggccgcca gacgactcat atcgtatttt ccttccgcga
     5041 tatcacttcc atgacgacag gatagtctga gggttatctg tcacagattt gagggtggtt
     5101 cgtcacattt gttctgacct actgagggta atttgtcaca gttttgctgt ttccttcagc
     5161 ctgcatggat tttctcatac tttttgaact gtaattttta aggaagccaa atttgagggc
     5221 agtttgtcac agttgatttc cttctctttc ccttcgtcat gtgacctgat atcgggggtt
     5281 agttcgtcat cattgatgag ggttgattat cacagtttat tactctgaat tggctatccg
     5341 cgtgtgtacc tctacctgga gtttttccca cggtggatat ttcttcttgc gctgagcgta
     5401 agagctatct gacagaacag ttcttctttg cttcctcgcc agttcgctcg ctatgctcgg
     5461 ttacacggct gcggcgagcg ctagtgataa taagtgactg aggtatgtgc tcttcttatc
     5521 tccttttgta gtgttgctct tattttaaac aactttgcgg ttttttgatg actttgcgat
     5581 tttgttgttg ctttgcagta aattgcaaga tttaataaaa aaacgcaaag caatgattaa
     5641 aggatgttca gaatgaaact catggaaaca cttaaccagt gcataaacgc tggtcatgaa
     5701 atgacgaagg ctatcgccat tgcacagttt aatgatgaca gcccggaagc gaggaaaata
     5761 acccggcgct ggagaatagg tgaagcagcg gatttagttg gggtttcttc tcaggctatc
     5821 agagatgccg agaaagcagg gcgactaccg cacccggata tggaaattcg aggacgggtt
     5881 gagcaacgtg ttggttatac aattgaacaa attaatcata tgcgtgatgt gtttggtacg
     5941 cgattgcgac gtgctgaaga cgtatttcca ccggtgatcg gggttgctgc ccataaaggt
     6001 ggcgtttaca aaacctcagt ttctgttcat cttgctcagg atctggctct gaaggggcta
     6061 cgtgttttgc tcgtggaagg taacgacccc cagggaacag cctcaatgta tcacggatgg
     6121 gtaccagatc ttcatattca tgcagaagac actctcctgc ctttctatct tggggaaaag
     6181 gacgatgtca cttatgcaat aaagcccact tgctggccgg ggcttgacat tattccttcc
     6241 tgtctggctc tgcaccgtat tgaaactgag ttaatgggca aatttgatga aggtaaactg
     6301 cccaccgatc cacacctgat gctccgactg gccattgaaa ctgttgctca tgactatgat
     6361 gtcatagtta ttgacagcgc gcctaacctg ggtatcggca cgattaatgt cgtatgtgct
     6421 gctgatgtgc tgattgttcc cacgcctgct gagttgtttg actacacctc cgcactgcag
     6481 tttttcgata tgcttcgtga tctgctcaag aacgttgatc ttaaagggtt cgagcctgat
     6541 gtacgtattt tgcttaccaa atacagcaat agcaatggct ctcagtcccc gtggatggag
     6601 gagcaaattc gggatgcctg gggaagcatg gttctaaaaa atgttgtacg tgaaacggat
     6661 gaagttggta aaggtcagat ccggatgaga actgtttttg aacaggccat tgatcaacgc
     6721 tcttcaactg gtgcctggag aaatgctctt tctatttggg aacctgtctg caatgaaatt
     6781 ttcgatcgtc tgattaaacc acgctgggag attagataat gaagcgtgcg cctgttattc
     6841 caaaacatac gctcaatact caaccggttg aagatacttc gttatcgaca ccagctgccc
     6901 cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc tcgcggtaat gccattactt
     6961 tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt gctccggggt gatagtgttg
     7021 agaagacctc tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt actgaggacg
     7081 cactggatga tctcatccct tcttttctac tgactggtca acagacaccg gcgttcggtc
     7141 gaagagtatc tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa gctgctgcac
     7201 ttaccgaaag tgattatcgt gttctggttg gcgagctgga tgatgagcag atggctgcat
     7261 tatccagatt gggtaacgat tatcgcccaa caagtgctta tgaacgtggt cagcgttatg
     7321 caagccgatt gcagaatgaa tttgctggaa atatttctgc gctggctgat gcggaaaata
     7381 tttcacgtaa gattattacc cgctgtatca acaccgccaa attgcctaaa tcagttgttg
     7441 ctcttttttc tcaccccggt gaactatctg cccggtcagg tgatgcactt caaaaagcct
     7501 ttacagataa agaggaatta cttaagcagc aggcatctaa ccttcatgag cagaaaaaag
     7561 ctggggtgat atttgaagct gaagaagtta tcactctttt aacttctgtg cttaaaacgt
     7621 catctgcatc aagaactagt ttaagctcac gacatcagtt tgctcctgga gcgacagtat
     7681 tgtataaggg cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca actgagtgta
     7741 tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc agcaccctga tgcgaccacg
     7801 ttttagtcta cgtttatctg tctttactta atgtcctttg ttacaggcca gaaagcataa
     7861 ctggcctgaa tattctctct gggcccactg ttccacttgt atcgtcggtc tgataatcag
     7921 actgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc
     7981 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
     8041 gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
     8101 catggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     8161 cgtcggtctg attattagtc tggaaccacg gtcccactcg tatcgtcggt ctgattatta
     8221 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacgatcc
     8281 cactcgtgtt gtcggtctga ttatcggtct gggaccacgg tcccacttgt attgtcgatc
     8341 agactatcag cgtgagacta cgattccatc aatgcctgtc aagggcaagt attgacatgt
     8401 cgtcgtaacc tgtagaacgg agtaacctcg gtgtgcggtt gtatgcctgc tgtggattgc
     8461 tgctgtgtcc tgcttatcca caacattttg cgcacggtta tgtggacaaa atacctggtt
     8521 acccaggccg tgccggcacg ttaaccgggc tgcatccgat gcaagtgtgt cgctgtcgac
     8581 gagctcgcga gctcggacat gaggttgccc cgtattcagt gtcgctgatt tgtattgtct
     8641 gaagttgttt ttacgttaag ttgatgcaga tcaattaata cgatacctgc gtcataattg
     8701 attatttgac gtggtttgat ggcctccacg cacgttgtga tatgtagatg ataatcatta
     8761 tcactttacg ggtcctttcc ggtgatccga caggttacgg ggcggcgacc tcgcgggttt
     8821 tcgctattta tgaaaatttt ccggtttaag gcgtttccgt tcttcttcgt cataacttaa
     8881 tgtttttatt taaaataccc tctgaaaaga aaggaaacga caggtgctga aagcgagctt
     8941 tttggcctct gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat ggaagtccga
     9001 gctcatcgct aataacttcg tatagcatac attatacgaa gttatattcg at
//
