pCASP vector (V008722)

LOCUS       40924_9056        3048 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCASP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Widmaier DM, Voigt CA.
  TITLE     Secreting Spider Silk in Salmonella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Widmaier DM, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2006) Pharmaceutical Chemistry, UCSF, 1700 4th 
            Street, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 3048)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3048)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2006) Pharmaceutical Chemistry, UCSF, 1700 4th Street, San 
            Francisco, CA 94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..170
                     /note="sicA promoter; psicA"
                     /regulatory_class="promoter"
     CDS             171..563
                     /codon_start=1
                     /gene="SicP"
                     /product="SicP"
                     /label=SicP
                     /note="secretion chaperone"
                     /protein_id="ABN48557.1"
                     /translation="MQAHQDIIANIGEKLGLPLTFDDNNQCLLLLDSDIFTSIEAKDDI
                     WLLNGMIIPLSPVCGDSIWRQIMVINGELAANNEGTLAYIDAAETLLLIHAITDLTNTY
                     HIISQLESFVNQQEALKNILQEYAKV"
     gene            171..563
                     /gene="SicP"
                     /label=SicP
     CDS             1054..1074
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     terminator      1146..1232
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1397..1985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2073..2167)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2191..2847)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2848..2950)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"