LOCUS       40924_9056        3048 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCASP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Widmaier DM, Voigt CA.
  TITLE     Secreting Spider Silk in Salmonella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Widmaier DM, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2006) Pharmaceutical Chemistry, UCSF, 1700 4th 
            Street, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 3048)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3048)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2006) Pharmaceutical Chemistry, UCSF, 1700 4th Street, San 
            Francisco, CA 94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..170
                     /note="sicA promoter; psicA"
                     /regulatory_class="promoter"
     CDS             171..563
                     /codon_start=1
                     /gene="SicP"
                     /product="SicP"
                     /label=SicP
                     /note="secretion chaperone"
                     /protein_id="ABN48557.1"
                     /translation="MQAHQDIIANIGEKLGLPLTFDDNNQCLLLLDSDIFTSIEAKDDI
                     WLLNGMIIPLSPVCGDSIWRQIMVINGELAANNEGTLAYIDAAETLLLIHAITDLTNTY
                     HIISQLESFVNQQEALKNILQEYAKV"
     gene            171..563
                     /gene="SicP"
                     /label=SicP
     CDS             1054..1074
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     terminator      1146..1232
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1397..1985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2073..2167)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2191..2847)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2848..2950)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgagccac aagaaaacga ggtacggcat tgagccgcgt aaggcagtag cgatgtattc
       61 attgggcgtt ttttgaatgt tcactaacca ccgtcggggt ttaataactg catcagataa
      121 acgcagtcgt taagttctac aaagtcggtg acagataaca ggagtaagta atgcaagcac
      181 accaggatat tatcgctaat attggtgaga aattgggttt accgctcact tttgacgaca
      241 acaatcagtg cttattatta ctcgatagcg atatttttac gtctattgaa gctaaagatg
      301 atatctggtt attgaacggt atgattatac cgttatcgcc tgtttgtggc gattctatct
      361 ggcggcagat tatggtgatt aatggtgaac tggctgcgaa taatgaaggt acgttagcgt
      421 atattgatgc cgcagagacg ttgttgctta tacatgcaat taccgatctg acaaatactt
      481 accatattat atcgcagctt gagtcatttg tgaatcagca ggaagcgctc aaaaacatac
      541 tgcaggaata tgctaaagta tgaggagaga aaattgaata atttaacgtt gtcttcgttt
      601 tcaaaagttg gtgtgtcgaa tgatgcccga ctttatattg ctaaggaaaa tactgataag
      661 gcatatgttg cgcctgaaaa attttcgtca aaagtattaa cctggcttgg aaaaatgccg
      721 ttatttaaaa acactgaagt ggtgcaaaaa catacggaaa atatcagagt acaggaccaa
      781 aagattttac agacatttct ccatgcacta acggaaaaat atggggaaac agcggttaat
      841 gacgcactgt taatgtcccg tataaatatg aacaaacccc ttacccaacg tttagcagtg
      901 cagatcacgg agtgtgtaaa agctgctgac gaagggttta taaaccttat taagagcaag
      961 gataatgttg gtgtcaggaa tgccgcttta gtcataaaag gcggcgatac aaaagtggca
     1021 gaaaaaaata acgatgttgg agcagaaagt ggagagaatt tgtattttca gtcaggaaag
     1081 cttggatccc tgcaggcctc agggcccgat cgatgcggcc gcttaattaa ttaatctaga
     1141 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1201 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag ggcgttcggc
     1261 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1321 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1381 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1441 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1501 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1561 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     1621 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1681 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1741 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1801 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1861 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1921 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1981 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2041 gttaagggat tttggtcatg actagtgctt ggattctcac caataaaaaa cgcccggcgg
     2101 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2161 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     2221 ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg
     2281 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2341 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2401 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2461 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2521 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2581 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     2641 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2701 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2761 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2821 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2881 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2941 gtgccgatca acgtctcatt ttcgccagat atcgacgtct aagaaaccat tattatcatg
     3001 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
