pCAM45 vector (V008772)

LOCUS       V008772                 8152 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008772
VERSION     V008772
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   May JP, Walker CA, Maskell DJ, Slater JD.
  TITLE     Development of an in vivo Himar1 transposon mutagenesis system for
            use in Streptococcus equi subsp. equi
  JOURNAL   FEMS Microbiol. Lett. 238 (2), 401-409 (2004)
   PUBMED   15358426
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   May JP, Maskell DJ, Slater JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2004) Clinical Veterinary Medicine, University of
            Cambridge, Madingley Road, Cambridge CB3 0ES, UK
REFERENCE   3  (bases 1 to 8152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2004"; volume: "238"; issue: "2"; pages:
            "401-409"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUL-2004) Clinical Veterinary Medicine, University of Cambridge,
            Madingley Road, Cambridge CB3 0ES, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     repeat_region   622..650
     CDS             864..1598
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     rep_origin      complement(1675..1993)
                     /direction=LEFT
                     /label="R6K gamma"
                     /note="R6K gamma"
     repeat_region   2028..2056
     primer_bind     2216..2232
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(2275..2291)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2299..2315)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2323..2353)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2368..2389)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2677..3265)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3764..3775)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             4036..5082
                     /codon_start=1
                     /product="mariner transposase C9 mutant"
                     /label="mariner transposase C9 mutant"
                     /protein_id="AAT79489.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDIKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             5443..5604
                     /codon_start=1
                     /gene="repC"
                     /product="RepC"
                     /label="repC"
                     /protein_id="AAT79490.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     gene            5443..5604
                     /gene="repC"
                     /label="repC"
     CDS             complement(6776..7567)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"