LOCUS       V008772                 8152 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008772
VERSION     V008772
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   May JP, Walker CA, Maskell DJ, Slater JD.
  TITLE     Development of an in vivo Himar1 transposon mutagenesis system for
            use in Streptococcus equi subsp. equi
  JOURNAL   FEMS Microbiol. Lett. 238 (2), 401-409 (2004)
   PUBMED   15358426
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   May JP, Maskell DJ, Slater JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2004) Clinical Veterinary Medicine, University of
            Cambridge, Madingley Road, Cambridge CB3 0ES, UK
REFERENCE   3  (bases 1 to 8152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2004"; volume: "238"; issue: "2"; pages:
            "401-409"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUL-2004) Clinical Veterinary Medicine, University of Cambridge,
            Madingley Road, Cambridge CB3 0ES, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     repeat_region   622..650
     CDS             864..1598
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     rep_origin      complement(1675..1993)
                     /direction=LEFT
                     /label="R6K gamma"
                     /note="R6K gamma"
     repeat_region   2028..2056
     primer_bind     2216..2232
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(2275..2291)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2299..2315)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2323..2353)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2368..2389)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2677..3265)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3764..3775)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             4036..5082
                     /codon_start=1
                     /product="mariner transposase C9 mutant"
                     /label="mariner transposase C9 mutant"
                     /protein_id="AAT79489.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDIKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             5443..5604
                     /codon_start=1
                     /gene="repC"
                     /product="RepC"
                     /label="repC"
                     /protein_id="AAT79490.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     gene            5443..5604
                     /gene="repC"
                     /label="repC"
     CDS             complement(6776..7567)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaata aacacgtggc gagaaaggaa
      421 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      481 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccactc
      541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      601 acgggataat accgcgccac ataacaggtt ggctgataag tccccggtct aacaaagaaa
      661 aacacatttt tttgtgaaaa ttcgttttta ttattcaaca tagttccctt caagagcgat
      721 acccctcgaa ttgacgcgaa ttcgcccttg atatcgaagc aaacttaaga gtgtgttgat
      781 agtgcagtat cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      841 tgtaattaag aaggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa
      901 cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg
      961 tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac
     1021 aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac
     1081 tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac
     1141 agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag
     1201 tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc
     1261 gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc
     1321 aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa
     1381 taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact
     1441 ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc
     1501 aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt
     1561 ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt
     1621 agatatcaag ggcgaattcg cgtcctcggt accgggcccc ccctcgaggt cgacatccct
     1681 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt
     1741 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt
     1801 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc
     1861 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaagcat
     1921 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgctg
     1981 atcttcggat ctactgcatt taatactagc gacgccatct atgtgtcaga ccggggactt
     2041 atcagccaac ctgttagcag aactttaaaa gtgctcatca ttggaaaaag gctgcgcaac
     2101 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2161 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2221 acgacggcca gtgagcgcgc gtaatacgac tcacgtttgc ttggcgtaat catggtcata
     2281 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2341 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2401 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2461 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3301 tcagtggaac gaaaactcac gttaagggat tttggtcatg aattcgagct cggtacccgg
     3361 ggatcgttaa atttatactg caatcggatg cgattattga ataaaagata tgagagattt
     3421 atctaatttc ttttttcttg taaaaaaaga aagttcttaa aggttttata gttttggtcg
     3481 tagagcacac ggtttaacga cttaattacg aagtaaataa gtctagtgtg ttagacttta
     3541 tgaaatctat atacgtttat atatatttat tatccgattt tttattaaaa cgtctcaaaa
     3601 tcgtttctga gacgttttag cgtttatttc gtttagttat cggcataatc gttaaaacag
     3661 gcgttatcgt agcgtaaaag cccttgagcg tagcgtggct ttgcagcgaa gatgttgtct
     3721 gttagattat gaaagccgat gactgaatga aataataagc gcagcgccct tctatttcgg
     3781 ttggaggagg ctcaagggag tatgagggaa tgaaattccc tcatgggttt gattttaaaa
     3841 attgcttgca attttgccga gcggtagctc gacgcaaaag ctcatacggt cttattttaa
     3901 catattgata attgtttgtc attgaattca gaaaaataat gaaaatagtt tactgataac
     3961 gcttccaata agaaaaaata aggaatttat ggtataatga aatctagtat tgactagaaa
     4021 aggagcattc ctcatatgga aaaaaaggaa tttcgtgttt tgataaaata ctgttttctg
     4081 aagggaaaaa atacagtgga agcaaaaact tggcttgata atgagtttcc ggactctgcc
     4141 ccagggaaat caacaataat tgattggtat gcaaaattca agcgtggtga aatgagcacg
     4201 gaggacggtg aacgcagtgg acgcccgaaa gaggtggtta ccgacgaaaa catcaaaaaa
     4261 atccacaaaa tgattttgaa tgaccgtaaa atgaagttga tcgagatagc agaggcctta
     4321 aagatatcaa aggaacgtgt tggtcatatc attcatcaat atttggatat gcggaagctc
     4381 tgtgcgaaat gggtgccgcg cgagctcaca tttgaccaaa aacaacgacg tgttgatgat
     4441 tctaagcggt gtttgcagct gttaactcgt aatacacccg agtttttccg tcgatatgtg
     4501 acaatggatg aaacatggct ccatcactac actcctgagt ccaatcgaca gtcggctgag
     4561 tggacagcga ccggtgaacc gtctccgaag cgtggaaaga ctcaaaagtc cgctggcaaa
     4621 gtaatggcct ctgttttttg ggatgcgcat ggaataattt ttatcgatta tcttgagaag
     4681 ggaaaaacca tcaacagtga ctattatatg gcgttattgg agcgtttgaa ggtcgaaatc
     4741 gcggcaaaac ggccccacat gaagaagaaa aaagtgttgt tccaccaaga caacgcaccg
     4801 tgccacaagt cattgagaac gatggcaaaa attcatgaat tgggcttcga attgcttccc
     4861 cacccgccgt attctccaga tctggccccc agcgactttt tcttgttctc agacatcaaa
     4921 aggatgctcg cagggaaaaa atttggctgc aatgaagagg tgatcgccga aactgaggcc
     4981 tattttgagg caaaaccgaa ggagtactac caaaatggta tcaaaaaatt ggaaggtcgt
     5041 tataatcgtt gtatcgctct tgaagggaac tatgttgaat aataagcatg ctaagggcga
     5101 attccagcac actggcggcc gttactagtg gatccgagct cggtaccaag ctgctggaaa
     5161 atttttgaaa aaaatttgga atttggaaaa aaatgggggg aaaggaagcg aattttgctt
     5221 ccgtactacg accccccatt aagtgccgag tgccaatttt tgtgccaaaa acgctctatc
     5281 ccaactggct caagggttta aggggttttt caatcgccaa cgaatcgcca acgttttcgc
     5341 caacgttttt tataaatcta tatttaagta gctttattgt tgtttttatg attacaaagt
     5401 gatacactaa ctttataaaa ttatttgatt ggagtttttt aaatggtgat ttcagaatcg
     5461 aaaaaaagag ttatgatttc tctgacaaaa gagcaagata aaaaattaac agatatggcg
     5521 aaacaaaaag gtttttcaaa atctgcggtt gcggcgttag ctatagaaga atatgcaaga
     5581 aaggaatcag aacaaaaaaa ataagcgaaa gctcgcgttt ttagaaggat acgagttttc
     5641 gctacttgtt tttgataagg taattatatc atggctatta aaaatactaa agctagaaat
     5701 tttggatttt tattatatcc tgactcaatt cctaatgatt ggaaagaaaa ttagagagtt
     5761 tgggcgtatc tatggctgtc agtcctttac acgatatgga cgaaaaaaaa gataaagata
     5821 catggaataa tagtaatatt atacaaaatg gaaagcacta taaaaaacca cactatcacg
     5881 ttatatatat tgcacgaaat cctgtaacaa tagaaagcgt taggaacaag attaagcgaa
     5941 aattggggaa tagttcagtt gctcatgttg agatacttga ttatatcaaa ggttcatatg
     6001 aatatttgac tcatgaatca aaggacgcta ttgctaagaa taaacatata tacgacaaaa
     6061 aagatatttt gaacattaat gattttgata ttgaccgcta tataacactt gatgaaagcc
     6121 aaaaaagaga attgaagaat ttacttttag atatagtgga tgactataat ttggtaaata
     6181 caaaagattt aatggctttt attcgcctta ggggagcgga gtttggaatt ttaaatacga
     6241 atgatgtaaa agatattgtt tcaacaaact ctagcgcctt tagattatgg tttgagggca
     6301 attatcagtg tggatataga gcaagttatg caaaggttct tgatgctgaa acgggggaaa
     6361 taaaatgaca aacaaagaaa aagagttatt tgctgaaaat gaggaattaa aaaaagaaat
     6421 taaggactta aaagagcgta ttgaaagata cagagaaatg gaagttgaat taagtacaac
     6481 aatagattta ttgagaggag ggattattga ataaataaaa gccccctgac gaaagtcgaa
     6541 gggggttttt attttggttt gatgttgcga ttaatagcaa tacaattgca ataaacaaaa
     6601 tgatcctcta gagtcgatac aaattcctcg taggcgctcg ggacccctat ctagcgaact
     6661 tttagaaaag atataaaaca tcagagtatg gacagttgcg gatgtacttc agaaaagatt
     6721 agatgtctaa aaagcttgta gttaaagctt tttagacatc taaatctagg tactaaaaca
     6781 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
     6841 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
     6901 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
     6961 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
     7021 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
     7081 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
     7141 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
     7201 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
     7261 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
     7321 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
     7381 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
     7441 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
     7501 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
     7561 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
     7621 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
     7681 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
     7741 tttgaaacca caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag
     7801 cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct
     7861 gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata
     7921 ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct
     7981 cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat
     8041 tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat
     8101 cgacccatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
