pCAhIFNGSTm vector (V008780)

LOCUS       V008780                 6738 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008780
VERSION     V008780
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6738)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..137
                     /codon_start=1
                     /note="unnamed protein product; hIFNG signal sequence"
                     /protein_id="SJL87797.1"
                     /translation="MKYTSYILAFQLCIVLGSLGCYC"
     CDS             149..1211
                     /codon_start=1
                     /note="unnamed protein product; mature hST6GalI mutant"
                     /protein_id="SJL87798.1"
                     /translation="GRHGV*FPVCILKQHPGPPQGPPDPRQSQRPSQGQTRGLLPGVEQ
                     GQLFQKPYP*AAKDLEELPKHEQVQSVLQGARTRHQVQCRGPALPPPGPCECIHGRGHR
                     FSLQYL*MGGLSAQGEH*DQGWALGQVCCCVVSGISEVLPTRQRNR*S*RSPEV*WGTH
                     SQLPTRCGHKNYHSPDELSVGYHREALPQRQFVQ*RNPNCMGPICIPLRYPKVVPESGL
                     *FL*QLQDLS*AAPQSALLHPQAPDALGAMGHSSRNLPRRDSAKPPILWDAWYHHHDDA
                     V*PGGYL*VPPIQAQD*RVLLLPEVLR*CLHDGCLPPAAL*EEFGEASQPGHR*GHLPA
                     WKSHTAWLPDHSLL"
     regulatory      150..159
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1265..1684
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Pan troglodytes. Accession#:
                     Q9TTB0"
     polyA_signal    2095..2150
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2511..2527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2535..2551)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2559..2589)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2603..2624)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2682..2878
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2884..3018
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3256..3844)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4018..4875)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4876..4980)
                     /label="AmpR promoter"
     enhancer        5011..5390
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5011..5388
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5392..5669
                     /label="chicken beta-actin promoter"
     intron          5670..6687
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"