LOCUS       V008780                 6738 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008780
VERSION     V008780
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6738)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..137
                     /codon_start=1
                     /note="unnamed protein product; hIFNG signal sequence"
                     /protein_id="SJL87797.1"
                     /translation="MKYTSYILAFQLCIVLGSLGCYC"
     CDS             149..1211
                     /codon_start=1
                     /note="unnamed protein product; mature hST6GalI mutant"
                     /protein_id="SJL87798.1"
                     /translation="GRHGV*FPVCILKQHPGPPQGPPDPRQSQRPSQGQTRGLLPGVEQ
                     GQLFQKPYP*AAKDLEELPKHEQVQSVLQGARTRHQVQCRGPALPPPGPCECIHGRGHR
                     FSLQYL*MGGLSAQGEH*DQGWALGQVCCCVVSGISEVLPTRQRNR*S*RSPEV*WGTH
                     SQLPTRCGHKNYHSPDELSVGYHREALPQRQFVQ*RNPNCMGPICIPLRYPKVVPESGL
                     *FL*QLQDLS*AAPQSALLHPQAPDALGAMGHSSRNLPRRDSAKPPILWDAWYHHHDDA
                     V*PGGYL*VPPIQAQD*RVLLLPEVLR*CLHDGCLPPAAL*EEFGEASQPGHR*GHLPA
                     WKSHTAWLPDHSLL"
     regulatory      150..159
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1265..1684
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Pan troglodytes. Accession#:
                     Q9TTB0"
     polyA_signal    2095..2150
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2511..2527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2535..2551)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2559..2589)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2603..2624)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2682..2878
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2884..3018
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3256..3844)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4018..4875)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4876..4980)
                     /label="AmpR promoter"
     enhancer        5011..5390
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5011..5388
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5392..5669
                     /label="chicken beta-actin promoter"
     intron          5670..6687
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg tcagcttgat acaagaacta ctgatttcaa cttctttggc ttaattctct
       61 cggaaacgat gaaatataca agttatatct tggcttttca gctctgcatc gttttgggtt
      121 ctcttggctg ttactgccag gacccagcgg ccgccatggg gtctgattcc cagtctgtat
      181 cctcaagcag cacccaggac ccccacaggg gccgccagac cctcggcagt ctcagaggcc
      241 tagccaaggc caaaccagag gcctccttcc aggtgtggaa caaggacagc tcttccaaaa
      301 accttatccc taggctgcaa aagatctgga agaattacct aagcatgaac aagtacaaag
      361 tgtcctacaa ggggccagga ccaggcatca agttcagtgc agaggccctg cgctgccacc
      421 tccgggacca tgtgaatgta tccatggtag aggtcacaga ttttcccttc aatacctctg
      481 aatgggaggg ttatctgccc aaggagagca ttaggaccaa ggctgggcct tggggcaggt
      541 gtgctgttgt gtcgtcagcg ggatctctga agtcctccca actaggcaga gaaatcgatg
      601 atcatgacgc agtcctgagg tttaatgggg cacccacagc caacttccaa caagatgtgg
      661 gcacaaaaac taccattcgc ctgatgaact ctcagttggt taccacagag aagcgcttcc
      721 tcaaagacag tttgtacaat gaaggaatcc taattgtatg ggacccatct gtataccact
      781 cagatatccc aaagtggtac cagaatccgg attataattt ctttaacaac tacaagactt
      841 atcgtaagct gcaccccaat cagccctttt acatcctcaa gccccagatg ccttgggagc
      901 tatgggacat tcttcaagaa atctccccag aagagattca gccaaacccc ccatcctctg
      961 ggatgcttgg tatcatcatc atgatgacgc tgtgtgacca ggtggatatt tatgagttcc
     1021 tcccatccaa gcgcaagact gacgtgtgct actactacca gaagttcttc gatagtgcct
     1081 gcacgatggg tgcctaccac ccgctgctct atgagaagaa tttggtgaag catctcaacc
     1141 agggcacaga tgaggacatc tacctgcttg gaaaagccac actgcctggc ttccggacca
     1201 ttcactgcta agcacaggct cctcactctt ctccatcagg cattaaatga atggtctctt
     1261 ggcctatgta aaagaagcag aaaaccttaa gaaatatttt aatgcaggtc attcagatgt
     1321 agcggataat ggaactcttt tcttaggcat tttgaagaat tggaaagagg agagtgacag
     1381 aaaaataatg cagagccaaa ttgtctcctt ttacttcaaa ctttttaaaa actttaaaga
     1441 tgaccagagc atccaaaaga gtgtggagac catcaaggaa gacatgaatg tcaagttttt
     1501 caatagcaac aaaaagaaac gagatgactt cgaaaagctg actaattatt cggtaactga
     1561 cttgaatgtc caacgcaaag caatacatga actcatccaa gtgatggctg aactgtcgcc
     1621 agcagctaaa acagggaagc gaaaaaggag tcagatgctg tttcgaggtc gaagagcatc
     1681 ccagtaatgg ttgtcctgcc tgcaatattt gaattttaaa tctaaatcta tttattaata
     1741 tttaacatta tttatatggg gaatatattt ttagactcat caatcaaata agtatttata
     1801 atagcaactt ttgtgtaatg aaaatgaata tctattaata tatgtattat ttataattcc
     1861 tatatcctgt gactgtctca cttaatcctt tgttttctga ctaattaggc aaggctatgt
     1921 gattacaagg ctttatctca ggggccaact aggcagccaa cctaagcaag acctaggctt
     1981 ttgcaaaaag ctcaagctcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     2041 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     2101 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     2161 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     2221 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     2281 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     2341 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     2401 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     2461 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     2521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2581 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2641 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     2701 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     2881 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2941 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3001 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3061 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
     3181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3241 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4921 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4981 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     5041 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     5101 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     5161 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5221 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     5281 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     5341 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     5401 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     5461 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
     5521 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
     5581 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     5641 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
     5701 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     5761 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     5821 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     5881 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     5941 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     6001 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     6061 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     6121 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     6181 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     6241 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     6301 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     6361 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     6421 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     6481 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     6541 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     6601 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     6661 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     6721 catcattttg gcaaagaa
//
