pCa4B vector (V008902)

LOCUS       40924_7966        8223 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCa4B, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Markstein M, Pitsouli C, Villalta C, Celniker SE, Perrimon N.
  TITLE     Exploiting position effects and the gypsy retrovirus insulator to 
            engineer precisely expressed transgenes
  JOURNAL   Nat. Genet. 40 (4), 476-483 (2008)
  PUBMED    18311141
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Markstein M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2008) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 8223)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2008"; volume: "40"; issue: "4"; pages: "476-483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JAN-2008) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    313..382
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     gene            645..4780
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(4791..5376)
                     /label=P element 5' end
     rep_origin      complement(5611..6199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6373..7230)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7231..7335)
                     /label=AmpR promoter
     misc_feature    complement(join(8137..8223,1..146))
                     /label=P element 3' end
                     /note="P element 3' end"