LOCUS       40924_7966        8223 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCa4B, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Markstein M, Pitsouli C, Villalta C, Celniker SE, Perrimon N.
  TITLE     Exploiting position effects and the gypsy retrovirus insulator to 
            engineer precisely expressed transgenes
  JOURNAL   Nat. Genet. 40 (4), 476-483 (2008)
  PUBMED    18311141
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Markstein M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2008) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 8223)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2008"; volume: "40"; issue: "4"; pages: "476-483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JAN-2008) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    313..382
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     gene            645..4780
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(4791..5376)
                     /label=P element 5' end
     rep_origin      complement(5611..6199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6373..7230)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7231..7335)
                     /label=AmpR promoter
     misc_feature    complement(join(8137..8223,1..146))
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 ttctgagtgt cgtattgagt ctgagtgaga cagcgatatg attgttgatt aacccttagc
       61 atgtccgtgg ggtttgaatt aactcataat attaattaga cgaaattatt tttaaagttt
      121 tatttttaat aatttgcgag tacgcaaagc ttgggctgca ggtcgacctc gaggcctcga
      181 gttaacgtta cgttaacgtt aacgttcgag gtcgactcta gaggatccag atccactagt
      241 gctgcatcca acgcgttggg agctctccgg atcaattcgg cttcaggtac cgtcgacgat
      301 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
      361 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
      421 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
      481 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
      541 cagcgggttc tcgacggtca cggcgggcat gtcgacaagc cgaattgatc cactagaagg
      601 cctaattcgc ggccgcggat ctgatcccgg gcgggtaccg aattctagta tgtatgtaag
      661 ttaataaaac ccttttttgg agaatgtaga tttaaaaaaa catatttttt ttttattttt
      721 tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg
      781 tatttgaagt accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt
      841 cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt
      901 ccgctatctc tttcgccacc gtttgtagcg ttacctagcg tcaatgtccg ccttcagttg
      961 cactttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa
     1021 agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt
     1081 ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa
     1141 gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac
     1201 aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg
     1261 caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaatcg
     1321 cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgtgctcgct atataagaca
     1381 atttttaaga tcatatcatg atcaagacat ctaaaggcat tcattttcga ctacattctt
     1441 ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa
     1501 taaaagtata aacctacttc gtaggatact tcgttttgtt cggggttaga tgagcataac
     1561 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggac gcttcgcaga
     1621 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctcctg ccacccagtc
     1681 cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc
     1741 acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca
     1801 accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc
     1861 tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt
     1921 ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt
     1981 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
     2041 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
     2101 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
     2161 aacggccagg gaacacctga ttttccaggc catggtgcgg atgccacgac atctgaccta
     2221 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
     2281 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
     2341 tctggcattc gcctccgagg cactaaccga tccgccgctt ctgatctgcg atgagcccac
     2401 ctccggactg gactcattta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
     2461 gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt
     2521 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
     2581 cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag cattacatta
     2641 catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact
     2701 tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg
     2761 ccaagatatg gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg
     2821 gccaccaaaa atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc
     2881 acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa
     2941 ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa
     3001 tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga
     3061 ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata
     3121 aataaatttc atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta
     3181 acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact
     3241 cacgcaagtg ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac
     3301 ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta
     3361 ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag
     3421 tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa
     3481 cgattgccga attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc
     3541 cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc
     3601 tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct
     3661 cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct
     3721 tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc
     3781 gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta
     3841 gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta
     3901 acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct
     3961 tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat
     4021 atatccgaaa taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta
     4081 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     4141 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     4201 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     4261 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     4321 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     4381 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     4441 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     4501 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     4561 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     4621 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     4681 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     4741 acatttaatt tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc
     4801 taaacgacgc atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt
     4861 aaacaatgaa caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta
     4921 ctattcaaac ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg
     4981 gttcaggctc tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg
     5041 tctgaccttt tgcaggtgca gccttccact gcgaatcttt aaagtgggta tcacaaattt
     5101 gggagttttc accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac
     5161 acttcggcac gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt
     5221 tcattttttt ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca
     5281 acctttcctc tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg
     5341 gctatcgacg ggaccacctt atgttatttc atcatgggcc agacccacgt agtccagcgg
     5401 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     5461 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     5521 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     5581 caccgagccc gagttcaaga agaaggcgtt tttccatagg ctccgccccc ctgacgagca
     5641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     5821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6961 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     7381 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     7441 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     7501 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     7561 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     7621 gtgtgaaata ccgcaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac
     7681 aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt
     7741 ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa
     7801 tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt
     7861 aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat
     7921 acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt
     7981 ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aacaagcagt
     8041 tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg
     8101 cgagcacccg gaagctcacg atgagaatgg ccagaccatg atgaaataac ataaggtggt
     8161 cccgtcggca agagacatcc acttaacgta tgcttgcaat aagtgcgagt gaaaggaata
     8221 gta
//
