pC1300intB-35SnosBK vector (V008964)

LOCUS       40924_7896        9946 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC1300intB-35SnosBK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Binary expression vector for use in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2004) Molecular Cell Biology, Institute of 
            Biology, Leiden University, Wassenaarseweg 64, Leiden 2333 AL, The 
            Netherlands
REFERENCE   3  (bases 1 to 9946)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2004) Molecular Cell Biology, Institute of Biology, Leiden 
            University, Wassenaarseweg 64, Leiden 2333 AL, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(48..300)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(318..356)
                     /note="multiple cloning site; SalI, EcoRI, XhoI, BglII,
                     KpnI, SpeI are unique"
     misc_feature    complement(358..413)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(433..777)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(837..853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1056..1080
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2380..3006
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3443..4507
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4576..4770
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5114..5254
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5440..6028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6118..6909)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7334..7358
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7436..7610)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(7661..8347)
                     /gene="hptII"
                     /number=1
     intron          complement(8348..8537)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(8538..8876)
                     /gene="hptII"
                     /number=2
     promoter        complement(8955..9632)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9823..9844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9859..9889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9897..9913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9921..9937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"