LOCUS       40924_7896        9946 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC1300intB-35SnosBK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Binary expression vector for use in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2004) Molecular Cell Biology, Institute of 
            Biology, Leiden University, Wassenaarseweg 64, Leiden 2333 AL, The 
            Netherlands
REFERENCE   3  (bases 1 to 9946)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2004) Molecular Cell Biology, Institute of Biology, Leiden 
            University, Wassenaarseweg 64, Leiden 2333 AL, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(48..300)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(318..356)
                     /note="multiple cloning site; SalI, EcoRI, XhoI, BglII,
                     KpnI, SpeI are unique"
     misc_feature    complement(358..413)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(433..777)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(837..853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1056..1080
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2380..3006
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3443..4507
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4576..4770
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5114..5254
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5440..6028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6118..6909)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7334..7358
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7436..7610)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(7661..8347)
                     /gene="hptII"
                     /number=1
     intron          complement(8348..8537)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(8538..8876)
                     /gene="hptII"
                     /number=2
     promoter        complement(8955..9632)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9823..9844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9859..9889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9897..9913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9921..9937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattaattc gagctcgcca attctcatgt ttgacagctt atcatcggat ctagtaacat
       61 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
      121 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
      181 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
      241 cgcaagaccg gcaacaggat tcaattttaa gaaactttat tgccaaatgt ttgaacgatc
      301 tgcagccggg cggccgcact agtgggtacc gagatctgct cgaggaattc gtcgactgta
      361 attgtaaata gtaattgtaa tgttgtttgt tgtttgttgt tgttggtaat tgttgaagga
      421 tctcaagaag cctgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
      481 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
      541 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg
      601 tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca
      661 atgatggcat ttgtaggagc caccttcctt ttctactgtc ctttcgatga agtgacagat
      721 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttaaa agtctcaata
      781 gccctctggt cttctgaggg gggatcctnt agagcctgca ggcatgcaag cttggcactg
      841 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      901 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      961 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
     1021 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     1081 ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat
     1141 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     1201 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     1261 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     1321 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     1381 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     1441 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     1501 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     1561 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     1621 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     1681 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     1741 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     1801 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     1861 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     1921 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     1981 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     2041 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     2101 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     2161 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     2221 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     2281 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     2341 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     2401 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     2461 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     2521 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     2581 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     2641 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     2701 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     2761 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     2821 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     2881 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     2941 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     3001 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     3061 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     3121 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     3181 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     3241 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     3301 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     3361 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     3421 aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     3481 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     3541 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     3601 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     3661 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     3721 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     3781 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     3841 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     3901 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     3961 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     4021 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     4081 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     4141 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     4201 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     4261 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     4321 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     4381 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     4441 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     4501 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     4561 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     4621 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     4681 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     4741 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     4801 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     4861 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     4921 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     4981 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5041 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5101 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     5161 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5221 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     5281 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5341 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5401 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5461 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5521 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5581 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5641 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5701 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5761 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5821 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5881 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5941 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6001 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6061 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa
     6121 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
     6181 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
     6241 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
     6301 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
     6361 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
     6421 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
     6481 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
     6541 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
     6601 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
     6661 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
     6721 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
     6781 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
     6841 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
     6901 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     6961 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     7021 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     7081 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     7141 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     7201 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     7261 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     7321 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     7381 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct
     7441 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
     7501 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
     7561 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgaagatc
     7621 cgtcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
     7681 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     7741 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     7801 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     7861 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
     7921 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     7981 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     8041 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     8101 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     8161 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     8221 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     8281 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     8341 cgaccggctg cagttatcat catcatcata gacacacgaa ataaagtaat cagattatca
     8401 gttaaagcta tgtaatattt acaccataac caatcaatta aaaaatagat cagtttaaag
     8461 aaagatcaaa gctcaaaaaa ataaaaagag aaaagggtcc taaccaagaa aatgaaggag
     8521 aaaaactaga aatttacctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     8581 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg
     8641 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
     8701 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     8761 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg
     8821 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct
     8881 cattgccccc ccggatctgc gaaagctcga cggatcttcg agagagatag atttgtagag
     8941 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
     9001 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
     9061 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     9121 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     9181 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     9241 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     9301 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     9361 tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     9421 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     9481 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     9541 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     9601 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     9661 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta
     9721 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9781 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9841 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9901 gagcggataa caatttcaca caggaaacag ctatgaccat gattac
//
