PC-GW-Bar vector (V008974)

Basic Vector Information

      • Vector Name:
      • PC-GW-Bar
      • Antibiotic Resistance:
      • Chloramphenicol
      • Length:
      • 11483 bp
      • Type:
      • Binary vector
      • Source/Author:
      • Dalal J, Yalamanchili R, La Hovary C, Ji M, Rodriguez-Welsh M, Aslett D, Ganapathy S, Grunden A, Sederoff H, Qu R.

PC-GW-Bar vector Vector Map

PC-GW-Bar11483 bp500100015002000250030003500400045005000550060006500700075008000850090009500100001050011000pVS1 StaApVS1 RepApVS1 oriVbomoriKanRLB T-DNA repeatCaMV poly(A) signalBlpRCaMV 35S promoter (enhanced)CAP binding sitelac promoterlac operatorM13 revCaMV 35S promoterattR1lac UV5 promoterCmRccdBattR235S terminator from pSIM1 cloning vectorM13 fwdRB T-DNA repeat

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

PC-GW-Bar vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_7756       11483 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector PC-GW-Bar, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Dalal J, Yalamanchili R, La Hovary C, Ji M, Rodriguez-Welsh M, 
            Aslett D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     A novel gateway-compatible binary vector series (PC-GW) for flexible
            cloning of multiple genes for genetic transformation of plants
  JOURNAL   Plasmid 81, 55-62 (2015)
  PUBMED    26188330
REFERENCE   2  (bases 1 to 11483)
  AUTHORS   Dalal J, Yalamanchili R, Hovary CL, Ji M, Rodriguez-Welsh M, Aslett 
            D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) Crop Science, North Carolina State 
            University, Campus Box 7287, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 11483)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11483)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 81,
            55-62 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2015) Crop Science, North Carolina State University, Campus 
            Box 7287, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2282..3346
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3415..3609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4957..5748)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6173..6197
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6459..7007)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(7052..7729)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    7920..7941
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7956..7986
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7994..8010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8018..8034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8651..8996
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    9085..9204
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9234..9264
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             9318..9974
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             10319..10621
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(10696..10820)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      10924..11149
                     /label=35S terminator from pSIM1 cloning vector
                     /note="35S terminator from pSIM1 cloning vector"
                     /regulatory_class="terminator"
     primer_bind     complement(11159..11175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11378..11402
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"

This page is informational only.