LOCUS       40924_7756       11483 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector PC-GW-Bar, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Dalal J, Yalamanchili R, La Hovary C, Ji M, Rodriguez-Welsh M, 
            Aslett D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     A novel gateway-compatible binary vector series (PC-GW) for flexible
            cloning of multiple genes for genetic transformation of plants
  JOURNAL   Plasmid 81, 55-62 (2015)
  PUBMED    26188330
REFERENCE   2  (bases 1 to 11483)
  AUTHORS   Dalal J, Yalamanchili R, Hovary CL, Ji M, Rodriguez-Welsh M, Aslett 
            D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) Crop Science, North Carolina State 
            University, Campus Box 7287, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 11483)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11483)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 81,
            55-62 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2015) Crop Science, North Carolina State University, Campus 
            Box 7287, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2282..3346
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3415..3609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4957..5748)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6173..6197
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6459..7007)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(7052..7729)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    7920..7941
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7956..7986
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7994..8010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8018..8034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8651..8996
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    9085..9204
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9234..9264
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             9318..9974
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             10319..10621
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(10696..10820)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      10924..11149
                     /label=35S terminator from pSIM1 cloning vector
                     /note="35S terminator from pSIM1 cloning vector"
                     /regulatory_class="terminator"
     primer_bind     complement(11159..11175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11378..11402
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
     6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     6421 ggaactactc acacattatt atggagaaac tcgagtcaaa tctcggtgac gggcaggacc
     6481 ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc atgccagttc
     6541 ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc
     6601 atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt gaagccctgt
     6661 gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg
     6721 cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg tgccttccag
     6781 gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag ccagggatag
     6841 cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg
     6901 aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc
     6961 gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatcga ttcctcgaga
     7021 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
     7081 ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     7141 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     7201 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     7261 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     7321 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     7381 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     7441 cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca cttgctttga
     7501 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     7561 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     7621 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     7681 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     7741 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     7801 gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7921 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7981 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8041 tacgaattcc gtaactataa cggtcctaag gtagcgaact tgatatctat gcgtatccgc
     8101 ctgatgaatg agttacgcta gggataacag ggtaatatag gaccatattg actacggctg
     8161 cagagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt
     8221 acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc
     8281 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
     8341 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     8401 ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat
     8461 caaaggcgat ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg
     8521 gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
     8581 tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc
     8641 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt
     8701 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat
     8761 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     8821 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     8881 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     8941 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag agaacacggc
     9001 tgcaggaaag acggttctag aatccgctta agacctccta ggtccaacgc gttttctact
     9061 agttacattt aaatatatag acgtccaagt ttgtacaaaa aagctgaacg agaaacgtaa
     9121 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     9181 tgtaaaacac aacatatcca gtcatattgg cggccgcatt aggcacccca ggctttacac
     9241 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta gtatgcgtcg agattttcag
     9301 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     9361 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     9421 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     9481 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gtatcccgta
     9541 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     9601 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     9661 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     9721 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     9781 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc acgatgggca
     9841 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     9901 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     9961 ggcagggcgg ggcgtaaacg cgtgcatccg gcttactaaa agccagataa cagtatgcgt
    10021 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
    10081 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
    10141 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
    10201 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
    10261 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
    10321 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
    10381 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
    10441 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
    10501 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
    10561 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
    10621 ataaatgtca ggctccctta tacacagcca gtcagcaggt cgaccatagt gactggatat
    10681 gttgtgtttt acagccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
    10741 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
    10801 gctttcttgt acaaagtggt ggatcccata tcatgtgtat tggcaaacag ctattatggg
    10861 tattatgggt aattcattgg ttcattatct atgtcgggtg cggagaaaga ggtaatgaaa
    10921 tggcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
    10981 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
    11041 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
    11101 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgaccta agcttggcac
    11161 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    11221 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    11281 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
    11341 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
    11401 acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt
    11461 atccgttcgt ccatttgtat gtg
//
