pBullet-vac-n vector (V008985)

LOCUS       V008985                 9181 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008985
VERSION     V008985
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A,
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM,
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
   PUBMED   24905498
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y.,
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD,
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 9181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        1406..1751
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2524
                     /gene="TIP1-1"
                     /label="Aquaporin TIP1-1"
                     /note="Aquaporin TIP1-1 from Arabidopsis thaliana.
                     Accession#: P25818"
     CDS             2537..3253
                     /label="ECFP"
                     /note="enhanced CFP"
     terminator      3275..3527
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3528..3694
                     /label="kan"
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3695..4486
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        4603..4707
                     /label="AmpR promoter"
     CDS             4708..5565
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      5574..5939
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5593..5937
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5940..5956
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5965..6681
                     /label="EYFP"
                     /note="enhanced YFP"
     protein_bind    6688..6812
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7244..7546)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7894..8550)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(8551..8653)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8831..8955)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      9020..9175
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"