LOCUS       V008985                 9181 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008985
VERSION     V008985
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A,
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM,
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
   PUBMED   24905498
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y.,
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD,
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 9181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        1406..1751
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1772..2524
                     /gene="TIP1-1"
                     /label="Aquaporin TIP1-1"
                     /note="Aquaporin TIP1-1 from Arabidopsis thaliana.
                     Accession#: P25818"
     CDS             2537..3253
                     /label="ECFP"
                     /note="enhanced CFP"
     terminator      3275..3527
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3528..3694
                     /label="kan"
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3695..4486
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        4603..4707
                     /label="AmpR promoter"
     CDS             4708..5565
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      5574..5939
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        5593..5937
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5940..5956
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5965..6681
                     /label="EYFP"
                     /note="enhanced YFP"
     protein_bind    6688..6812
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7244..7546)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7894..8550)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(8551..8653)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8831..8955)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      9020..9175
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatgccgatc agaaacatcg ccattggccg
     1801 tccagatgaa gccacccgtc ccgatgcctt aaaggcggcg ttggctgagt tcatttcaac
     1861 tttgatcttt gtcgtcgccg gttcaggctc tggcatggct ttcaacaagc tcactgaaaa
     1921 cggagccacc actccttctg gtctcgtagc tgctgcagtg gctcatgcct ttggactctt
     1981 cgtcgctgtc tcagttggtg ccaacatctc tggtggacac gttaaccctg ccgtcacttt
     2041 cggtgctttc attggtggta acatcactct cctccgtggt atcctctact ggattgctca
     2101 gcttctcggc tccgtcgtcg cttgcctcat ccttaaattc gccaccggtg gcttggctgt
     2161 gccggctttt ggtctctctg ctggagtagg agtgttgaac gctttcgttt tcgagatcgt
     2221 gatgacattc gggcttgttt acaccgtcta cgctacagcc attgacccca aaaacgggag
     2281 tcttggaaca attgctccca tcgcaatcgg tttcattgtt ggagccaaca tcttagctgg
     2341 aggagctttc tctggagcct ccatgaatcc cgccgtggct ttcggaccag cggtggtgag
     2401 ctggacatgg accaaccact gggtctactg ggccggacct ctcgtcggcg gtggaatcgc
     2461 tggactcatc tacgaagttt tcttcatcaa caccacacac gagcagctcc caaccacaga
     2521 ctaccccggg ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2581 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2641 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2701 cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta
     2761 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2821 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2881 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2941 caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc
     3001 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     3061 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3121 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     3181 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3241 cgagctgtac aagtaaagcg gcggcccggc tgcagatcgt tcaaacattt ggcaataaag
     3301 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     3361 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     3421 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     3481 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatccga tgataagctg
     3541 tcaaacatga gagctcccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     3601 ctgcaaagta aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag
     3661 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     3721 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3781 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3841 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     3901 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     3961 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4021 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4081 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4141 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4201 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4261 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4321 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4381 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4441 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgac acgtgttaat
     4501 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4561 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4921 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5221 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaagt taacagaaga
     5581 ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     5641 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa
     5701 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg acagtggtcc
     5761 caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc
     5821 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     5881 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacctc
     5941 gaggtcgacg gtatcgataa gcttatggtg agcaagggcg aggagctgtt caccggggtg
     6001 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6061 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6121 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     6181 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6241 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6301 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6361 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     6421 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     6481 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     6541 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     6601 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     6661 ggcatggacg agctgtacaa gcatatgaca agtttgtaca aaaaagctga acgagaaacg
     6721 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     6781 tactgtaaaa cacaacatat ccagtcacta tgaatcaact acttagatgg tattagtgac
     6841 ctgtagtcga ccgacagcct tccaaatgtt cttcgggtga tgctgccaac ttagtcgacc
     6901 gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt
     6961 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt
     7021 gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc
     7081 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc
     7141 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt
     7201 atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
     7261 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
     7321 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
     7381 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
     7441 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
     7501 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
     7561 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
     7621 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
     7681 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
     7741 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
     7801 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
     7861 ggcttttagt aagccggatc cacgcggcgt ttacgccccg ccctgccact catcgcagta
     7921 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
     7981 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     8041 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     8101 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     8161 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     8221 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     8281 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     8341 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     8401 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     8461 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     8521 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     8581 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     8641 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     8701 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
     8761 gcaaagtgcg tcgggtgatg ctgccaactt agtcgactac aggtcactaa taccatctaa
     8821 gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     8881 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     8941 cttgtacaaa gtggtctgat ctagagtccg caaaaatcac cagtctctct ctacaaatct
     9001 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     9061 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     9121 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgaagcgc
     9181 t
//
