pBullet-er-n vector (V008991)

LOCUS       40924_7651        8530 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-er-n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1865..2581
                     /label=ECFP
                     /note="enhanced CFP"
     misc_feature    2591..2602
                     /label=Region: ER retention signal
                     /note="Region: ER retention signal"
     terminator      2624..2876
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2877..3043
                     /label=kan
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3044..3835
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3952..4056
                     /label=AmpR promoter
     CDS             4057..4914
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4923..5288
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        4942..5286
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5289..5305
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5314..6030
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    6037..6161
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6593..6895)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7243..7899)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7900..8002)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8180..8304)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      8369..8524
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"