LOCUS       40924_7651        8530 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBullet-er-n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood JL.
  TITLE     The plant glycosyltransferase clone collection for functional 
            genomics
  JOURNAL   Plant J. 79 (3), 517-529 (2014)
  PUBMED    24905498
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   Lao J, Oikawa A, Bromley JR, Smith-Moritz AM, Chiu T-Y., 
            Christiansen KM, Hansen SF, Suttangkakul A, Vega-Sanchez ME, Yang F,
            Stonebloom S, Ebert B, Morrison S, McInerney P, Hadi MZ, Adams PD, 
            Ronald PC, Loque D, Scheller HV, Heazlewood JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley
            National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 8530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2014"; volume: "79"; issue: "3"; pages: "517-529"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2014) Joint BioEnergy Institute, Lawrence Berkeley National 
            Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      177..765
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1406..1751
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1865..2581
                     /label=ECFP
                     /note="enhanced CFP"
     misc_feature    2591..2602
                     /label=Region: ER retention signal
                     /note="Region: ER retention signal"
     terminator      2624..2876
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2877..3043
                     /label=kan
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             3044..3835
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3952..4056
                     /label=AmpR promoter
     CDS             4057..4914
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      4923..5288
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="promoter"
     promoter        4942..5286
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5289..5305
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5314..6030
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    6037..6161
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6593..6895)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7243..7899)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7900..8002)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(8180..8304)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      8369..8524
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 acgcgtctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       61 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgcg gccgcgagct ccctacccct
     1021 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1081 aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1141 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1201 ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
     1321 tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat gtcaaagata
     1381 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
     1441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1501 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
     1561 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1621 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     1681 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1741 tggagaggac agcccagatc tttgtttggg aatgaaggta caggagggtt tgttcgtggt
     1801 ggctgttttc taccttgctt atacgcagct agtcaagggg caacctcgca aggagtgcgg
     1861 atccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1921 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1981 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2041 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     2101 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2161 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2221 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2281 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     2341 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2401 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2461 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2521 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2581 gaagagatct catgacgagc tgtaaagcgg cggcccggct gcagatcgtt caaacatttg
     2641 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     2701 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     2761 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     2821 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatccgat
     2881 gataagctgt caaacatgag agctcccgga attgccagct ggggcgccct ctggtaaggt
     2941 tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag
     3001 gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3061 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3121 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3181 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3241 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3301 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3361 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3421 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3481 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3541 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     3601 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3661 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3721 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3781 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaca
     3841 cgtgttaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3901 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3961 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4021 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4081 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4141 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4201 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4261 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4321 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4381 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4441 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4501 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4561 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4621 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4681 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4741 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4801 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4861 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaagtt
     4921 aacagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     4981 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
     5041 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
     5101 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
     5161 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
     5221 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     5281 gaggacctcg aggtcgacgg tatcgataag cttatggtga gcaagggcga ggagctgttc
     5341 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5401 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5461 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     5521 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5581 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5641 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5701 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5761 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5821 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5881 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     5941 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     6001 atcactctcg gcatggacga gctgtacaag catatgacaa gtttgtacaa aaaagctgaa
     6061 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     6121 actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt
     6181 attagtgacc tgtagtcgac cgacagcctt ccaaatgttc ttcgggtgat gctgccaact
     6241 tagtcgaccg acagccttcc aaatgttctt ctcaaacgga atcgtcgtat ccagcctact
     6301 cgctattgtc ctcaatgccg tattaaatca taaaaagaaa taagaaaaag aggtgcgagc
     6361 ctcttttttg tgtgacaaaa taaaaacatc tacctattca tatacgctag tgtcatagtc
     6421 ctgaaaatca tctgcatcaa gaacaatttc acaactctta tacttttctc ttacaagtcg
     6481 ttcggcttca tctggatttt cagcctctat acttactaaa cgtgataaag tttctgtaat
     6541 ttctactgta tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     6601 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     6661 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     6721 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     6781 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     6841 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     6901 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     6961 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     7021 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     7081 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     7141 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     7201 ctgttatctg gcttttagta agccggatcc acgcggcgtt tacgccccgc cctgccactc
     7261 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc
     7321 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     7381 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     7441 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     7501 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     7561 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     7621 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     7681 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     7741 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     7801 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     7861 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     7921 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     7981 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     8041 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     8101 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta gtcgactaca ggtcactaat
     8161 accatctaag tagttgattc atagtgactg gatatgttgt gttttacagt attatgtagt
     8221 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     8281 ttcagctttc ttgtacaaag tggtctgatc tagagtccgc aaaaatcacc agtctctctc
     8341 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     8401 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     8461 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     8521 gtgaagcgct
//
