pBS-35S-Rluc vector (V009085)

LOCUS       40924_7131        5005 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBS-35S-Rluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Subramanian C, Kim BH, Lyssenko NN, Xu X, Johnson CH, von Arnim AG.
  TITLE     The Arabidopsis repressor of light signaling, COP1, is regulated by 
            nuclear exclusion: mutational analysis by bioluminescence resonance 
            energy transfer
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (17), 6798-6802 (2004)
  PUBMED    15084749
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   von Arnim A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Botany, University of Tennessee, 1406 Circle
            Drive, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 5005)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "17"; pages:
            "6798-6802"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2002) Botany, University of Tennessee, 1406 Circle Drive, 
            Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1460..1602
                     /note="translational enhancer; 5'UTR from tobacco etch
                     virus"
     CDS             1603..2535
                     /label=hRluc
                     /note="Renilla luciferase"
     regulatory      2548..2758
                     /gene="RLuc"
                     /label=cauliflower mosaic virus 35S terminator
                     /note="cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     promoter        complement(2820..2838)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2859..2875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2883..2899)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2907..2937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2952..2973)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3261..3849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4023..4880)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4881..4985)
                     /label=AmpR promoter